Crook:Media

BSE medium (1 L)

• 1 L dH₂O
• 5 g succinate
• 500 mg yeast extract
• 400 mg K₂HPO₄
• 400 mg KH₂PO₄
• 200 mg MgSO₄·7H₂O
• pH to 6.5
  • 3 g agar per 250 mL of broth for agar
  • 1 g agar per 250 mL of broth for top agar
  • 550 mg agar per 250 mL of broth for swarm agar

Autoclave
SOURCE: [1]

LB-Miller medium (1 L)

• 1 L dH₂O
• 10 g tryptone
• 5 g yeast extract
• 10 g NaCl
• 1 mL 2N NaOH
  • 3 g agar per 250 mL of broth for agar
  • 1 g agar per 250 mL of broth for top agar
  • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

LB-Lennox medium (1 L)

• 1 L dH₂O
• 10 g tryptone
• 5 g yeast extract
• 5 g NaCl
• 1 mL 2N NaOH
  • 3 g agar per 250 mL of broth for agar
  • 1 g agar per 250 mL of broth for top agar
  • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

LB-Luria medium (1 L)

• 1 L dH₂O
• 10 g tryptone
• 5 g yeast extract
• 500 mg NaCl
• 1 mL 2N NaOH
  • 3 g agar per 250 mL of broth for agar
  • 1 g agar per 250 mL of broth for top agar
  • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

PH medium (1 L)

• 1 L dH₂O
• 4 g peptone
• 0.5 g yeast extract
• 0.5 g tryptone
• 0.5 g CaCl₂·2H₂O
• 0.2 g MgSO₄·7H₂O
  • 3 g agar per 250 mL of broth for agar
  • 1 g agar per 250 mL of broth for top agar
  • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

PSY medium (1 L)

• 1 L dH₂O
• 20 g peptone
• 20 g sucrose
• 5 g yeast extract
  • 3 g agar per 250 mL of broth for agar
  • 1 g agar per 250 mL of broth for top agar
  • 550 mg agar per 250 mL of broth for swarm agar

Autoclave NOTE: For culturing Bradyrhizobium japonicum, supplement with 0.1% arabinose

SOB medium (1 L)

• 20 g tryptone
• 5 g yeast extract
• 600 mg NaCl
• 500 mg KCl
• 20 mL 1 M Glucose stock
• 10 mL 2 M Mg²⁺ stock
  • 3 g agar per 250 mL of broth for agar
  • 1 g agar per 250 mL of broth for top agar
  • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

SOC medium (1 L)

• 20 g tryptone
• 5 g yeast extract
• 600 mg NaCl
• 500 mg KCl
• 20 mL 1 M Glucose stock
• 10 mL 2 M Mg²⁺ stock
  • 3 g agar per 250 mL of broth for agar
  • 1 g agar per 250 mL of broth for top agar
  • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

TY medium(1 L)

• 1 L dH₂O
• 6 g tryptone
• 3 g yeast extract
• 0.5 g CaCl₂·2H₂O (this is equal to 3.4 mM)
  • 3 g agar per 250 mL of broth for agar
  • 1 g agar per 250 mL of broth for top agar
  • 550 mg agar per 250 mL of broth for swarm agar

Autoclave

YEM medium (1 L)

• 1 L dH₂O
• 10 g mannitol
• 1 g yeast extract
• 500 mg K₂HPO₄
• 200 mg MgSO₄·7H₂O
• 100 mg NaCl
  • 3 g agar per 250 mL of broth for agar
  • 1 g agar per 250 mL of broth for top agar
  • 550 mg agar per 250 mL of broth for swarm agar

Autoclave