Crook:Media
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BSE medium (1 L)
- 1 L dH2O
- 5 g succinate
- 500 mg yeast extract
- 400 mg K2HPO4
- 400 mg KH2PO4
- 200 mg MgSO4·7H2O
- pH to 6.5 with HCl
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave
SOURCE: [1]
DYGS medium (1 L)
- 1 L dH2O
- 2 g glucose
- 2 g yeast extract
- 1.5 g peptone
- 1.3 g glutamic acid
- 500 mg K2HPO4
- 500 mg MgSO4.7H2O
- pH to 6.0 with HCl
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave
SOURCE: [2]
LB-Miller medium (1 L)
- 1 L dH2O
- 10 g tryptone
- 5 g yeast extract
- 10 g NaCl
- 1 mL 2N NaOH
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave
LB-Lennox medium (1 L)
- 1 L dH2O
- 10 g tryptone
- 5 g yeast extract
- 5 g NaCl
- 1 mL 2N NaOH
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave
LB-Luria medium (1 L)
- 1 L dH2O
- 10 g tryptone
- 5 g yeast extract
- 500 mg NaCl
- 1 mL 2N NaOH
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave
PH medium (1 L)
- 1 L dH2O
- 4 g peptone
- 0.5 g yeast extract
- 0.5 g tryptone
- 0.5 g CaCl2·2H2O
- 0.2 g MgSO4·7H2O
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave
PSY medium (1 L)
- 1 L dH2O
- 20 g peptone
- 20 g sucrose
- 5 g yeast extract
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave
NOTE: For culturing Bradyrhizobium japonicum, supplement with 0.1% arabinose
PY medium (1 L)
- 1 L dH2O
- 5 g peptone
- 3 g yeast extract
- 1 g CaCl2·2H2O
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave
SOURCE: [3]
SOB medium (1 L)
- 20 g tryptone
- 5 g yeast extract
- 600 mg NaCl
- 500 mg KCl
- 20 mL 1 M Glucose stock
- 10 mL 2 M Mg2+ stock
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave
SOC medium (1 L)
- 20 g tryptone
- 5 g yeast extract
- 600 mg NaCl
- 500 mg KCl
- 20 mL 1 M Glucose stock
- 10 mL 2 M Mg2+ stock
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave
TY medium(1 L)
- 1 L dH2O
- 6 g tryptone
- 3 g yeast extract
- 0.5 g CaCl2·2H2O (this is equal to 3.4 mM)
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave
YEM medium (1 L)
- 1 L dH2O
- 10 g mannitol
- 1 g yeast extract
- 500 mg K2HPO4
- 200 mg MgSO4·7H2O
- 100 mg NaCl
- 3 g agar per 250 mL of broth for agar
- 1 g agar per 250 mL of broth for top agar
- 550 mg agar per 250 mL of broth for swarm agar
Autoclave