Common primer sequences: Difference between revisions
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* M13 forward | * M13 forward sequencing primer (-20): GTAAAACGACGGCCAGT | ||
* M13 forward sequencing primer (-40): GTTTTCCCAGTCACGAC | * M13 forward sequencing primer (-40): GTTTTCCCAGTCACGAC | ||
* M13 forward sequencing primer (-47): CGCCAGGGTTTTCCCAGTCACGAC | * M13 forward sequencing primer (-47): CGCCAGGGTTTTCCCAGTCACGAC | ||
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* T3 promoter: ATTAACCCTCACTAAAGGGA | * T3 promoter: ATTAACCCTCACTAAAGGGA | ||
* T7 universal primer: TAATACGACTCACTATAGGG | * T7 universal primer: TAATACGACTCACTATAGGG | ||
* T7 Terminator: GCTAGTTATTGCTCAGCGG | |||
* SP6 promoter: CATACGATTTAGGTGACACTATAG | * SP6 promoter: CATACGATTTAGGTGACACTATAG | ||
* SP6 universal primer: ATTTAGGTGACACTATAG | * SP6 universal primer: ATTTAGGTGACACTATAG | ||
* VF2: tgccacctgacgtctaagaa | * VF2: tgccacctgacgtctaagaa | ||
* VR: attaccgcctttgagtgagc | * VR: attaccgcctttgagtgagc | ||
Latest revision as of 14:48, 21 August 2007
- M13 forward sequencing primer (-20): GTAAAACGACGGCCAGT
- M13 forward sequencing primer (-40): GTTTTCCCAGTCACGAC
- M13 forward sequencing primer (-47): CGCCAGGGTTTTCCCAGTCACGAC
- M13 reverse sequencing primer: (-24): AACAGCTATGACCATG
- M13 reverse sequencing primer: (-48): AGCGGATAACAATTTCACACAGGA
- T3 promoter: ATTAACCCTCACTAAAGGGA
- T7 universal primer: TAATACGACTCACTATAGGG
- T7 Terminator: GCTAGTTATTGCTCAGCGG
- SP6 promoter: CATACGATTTAGGTGACACTATAG
- SP6 universal primer: ATTTAGGTGACACTATAG
- VF2: tgccacctgacgtctaagaa
- VR: attaccgcctttgagtgagc
