Cell and tissue lysis hub

This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.

Comparison of lysis methods

Sonication

• most efficient method of cell fractionation
• problem: heat build up which can denature proteins (proportionate to length of sonication)
• precaution: do on ice and sonicate intermittantly

Homogenisation

• best for animal tissue; less suitable for cells
• precaution: do on ice to reduce heat build-up and denaturation

Freeze-thaw

• least effective method
• plus: does not denature proteins as much as other methods

Detergents

• chemical method of lysis
• problems: detergent may inhibit subsequent reactions
• problems: detergent may disrupts protein interactions

Specific protocols

• Silver: Lysate for Western - Silver Lab: cell lysis for Western (method: Triton)
• Sauer:Lysing E. coli with Lysozymes - Sauer Lab: bacteria lysis (method: lysozyme)
• Blackburn:Yeast Colony PCR - Blackburn Lab: yeast lysis for colony PCR (method NaOH)
• Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer - Jacobs Lab: cell line lysis for protein (method: RIPA buffer)
• Streptomyces:Protocols/Mini-Maxi Prep - Streptomyces community, East Anglia: bacteria lysis for DNA (method: NaOH)

Related discussions on BioForum

• cell lysis methods comparison
• what solution to use for cell lysis