Bromophenol blue

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Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.
Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.
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* 500bp at 1% agarose
+
* 1000bp at 0.7% agarose
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* 500bp at 1%
* 150bp at 2%
* 150bp at 2%
* 50bp at 3%  
* 50bp at 3%  

Current revision

Bromophenol blue (BPB) is most commonly used as an agarose gel electrophoresis size marker. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than Xylene cyanol (539g/mol) because of its larger negative charge (hydroxyl groups).

Bromophenol blue is also a pH indicator which is yellow below pH=3 and blue-purple at more basic pH.

  • 1000bp at 0.7% agarose
  • 500bp at 1%
  • 150bp at 2%
  • 50bp at 3%

See also

External links

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