Bromodeoxyuridine (BrdU)
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'''Bromodeoxyuridine''' (5-bromo-2-deoxyuridine, '''BrdU''') is a synthetic nucleoside analogue of thymidine. BrdU is commonly used to detect cell proliferation. | '''Bromodeoxyuridine''' (5-bromo-2-deoxyuridine, '''BrdU''') is a synthetic nucleoside analogue of thymidine. BrdU is commonly used to detect cell proliferation. | ||
| + | == Mechanism == | ||
| + | BrdU is phosphorylated by cells to BrdUTP. This precursor is incorporated into newly synthesised DNA during S phase instead of deoxythymidine triphosphate (dTTP). It can then be detected with an anti-BrdU antibody coupled to a fluorophore or an enzyme. Other detection methods include BrdU quenching of DNA fluorophores and selective photolysis of BrdU-containing DNA. | ||
| + | |||
| + | == Examples == | ||
| + | * [[User:Mar/Notebook/2007-9-20|BrdU application in E16 mouse embryos]] | ||
== See also == | == See also == | ||
| + | * [[20.109(S08):Testing cell viability (Day3)|Fundamental bioengineering course notes]] mention BrdU in the introduction | ||
| + | * [http://en.wikipedia.org/wiki/BrdU bromodeoxyuridine (BrdU)], [http://en.wikipedia.org/wiki/Deoxyuridine deoxyuridine (dU)], [http://en.wikipedia.org/wiki/Deoxythymidine deoxythymidine (dT)] at the Wikipedia | ||
| - | + | [[Category:Material]] | |
| + | [[Category:DNA]] | ||
| + | [[Category:Chemical]] | ||
Current revision
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside analogue of thymidine. BrdU is commonly used to detect cell proliferation.
Mechanism
BrdU is phosphorylated by cells to BrdUTP. This precursor is incorporated into newly synthesised DNA during S phase instead of deoxythymidine triphosphate (dTTP). It can then be detected with an anti-BrdU antibody coupled to a fluorophore or an enzyme. Other detection methods include BrdU quenching of DNA fluorophores and selective photolysis of BrdU-containing DNA.
Examples
See also
- Fundamental bioengineering course notes mention BrdU in the introduction
- bromodeoxyuridine (BrdU), deoxyuridine (dU), deoxythymidine (dT) at the Wikipedia
