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<div style="background: url(/images/4/46/Fitaoback.png);">
 
 
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<hr>
<br>


<div id="header"><h1><p style="background:#CCFFFF; color:purple;"><font face=cursive size="6"><B>  Team FIT </font> </B></p></a></h1></div>


<!-- ======================== -->
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<!-- ======================== -->
<div class="menubar">
<ul id="menu" style="list-style:none;">
<li><a href="#">TOP</a></li>
<li><a href="fit_Introduction.html">Intro</a>
<ul class="sub"style="list-style:none;">
<li><a href="fit_Introduction.html#How">How to play kurohige</a></li>
<li><a href="fit_Introduction.html#Why">Why kurhige</a></li>
<li><a href="fit_Introduction.html#Pro">problem</a></li>
</ul>
</li>
<li><a href="fit_Approach and Goals.html">Approach</a>
<ul class="sub" style="list-style:none;">
<li><a href="fit_Approach and Goals.html#DNA">DNA</a></li>
<li><a href="fit_Approach and Goals.html#Silica">Silica</a></li>
<li><a href="fit_Approach and Goals.html#Fluorescence">Fluorescence</a></li>
</ul>
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<li><a href="fit_Method.html#DNA">DNA</a></li>
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<li><a href="fit_Method.html#Fluorescence">Fluorescence</a></li>
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</ul>
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<li><a href="fit_Results and Discussion.html#DNA">DNA</a></li>
<li><a href="fit_Results and Discussion.html#Silica">Silica</a></li>
</ul>
</li>
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<!-- ========== -->
<!-- ▲ここまで -->
<!-- ========== -->


  <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#home">Home</a>


<h2 p style="background:#FFFFFF; color:#003366;"><font face=cursive size="5"><B> Abstract  </font></B></p></h2>
    <ul>


<div style="padding: 35px;">
      <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#abs">Abstract</a></li>
      <li><a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#vid">Video</a></li>
 
    </ul>
  </li>
  <li><a href="fit_Introduction.html#pro">Projects</a>
    <ul>
      <li><a href="fit_Introduction.html#back">background & Motivation</a></li>
      <li><a href="fit_Introduction.html#goal">Goals</a></li>
 
    </ul>
  </li>
  <li><a href="fit_Approach and Goals.html#des">Design</a>
    <ul>
      <li><a href="fit_Approach and Goals.html#ear">Early Design</a></li>
      <li><a href="fit_Approach and Goals.html#fin">Final Design</a></li>
     
    </ul>
  </li>
  <li><a href="fit_Method.html#met">Method</a>
    <ul>
      <li><a href="fit_Method.html#a">Barrel particle</a></li>
      <li><a href="fit_Method.html#b">DNA modified</a></li>
      <li><a href="fit_Method.html#c">樽と人形の結合</a></li>
　　　<li><a href="fit_Method.html#d">樽人形剣</a></li>
　　　<li><a href="fit_Method.html#e">Material</a></li>
  </ul>
  </li>
  <li><a href="fit_Results and Discussion.html#">Result and Discassions</a>
    <ul>
      <li><a href="fit_Results and Discussion.html#a">Barrel particle and Doll particle</a></li>
      <li><a href="fit_Results and Discussion.html#b">DNA modified</a></li>
      <li><a href="fit_Results and Discussion.html#c">樽と人形の結合</a></li>
      <li><a href="fit_Results and Discussion.html#d">樽人形剣</a></li>
　　　<li><a href="fit_Results and Discussion.html#e">Conclusion</a></li>
    </ul>
  </li>
  <li><a href="fit_Member.html#team">Team</a>
    <ul>
      <li><a href="fit_Member.html#men">Menber</a></li>
      <li><a href="fit_Member.html#spo">Sponsor</a></li>
     
    </ul>
  </li>
</ul>
 
 
 
 
<div align="center">
<font size="6" color="#000022" face="Arial"><b>Abstract</b></font>
 
<font size="4" color="#000022" face="Arial">


<p>
<p>
<h3>&nbsp; We create “nano Pop-up Pirate “using by the complementary DNA base-pairing. Pop-up Pirate is a famous Japanese toy. A doll is sitting on the Barrel. Players stab the sword to when player stabs to the specific point, the doll will fly away from a barrel.
&nbsp; aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa<br>
bbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbb<br>
 
<br><br><br><br><br>
</p>
 
</div>
</font>
 


<br>
　Fluorescence labeled DNA was bound to the porous silica particle, of the barrel. And different fluorescence labeled mismatched DNA who is a doll in bound to the Barrel DNA. When mismatched DNA that is a sward in added, mismatched DNA would be released.
<br>
　Two fluorescence reagents are constructed the FRET system, and used in the detection of the states of DNAs. This system is applicable for the Drug Delivery systems .A porous silica is a container .DNA system is a drug release regulation. Free system is a drug release detection.</h3></p></div>


<font size="3" color="#000022" face="Arial">


<center>
<center>
<img alt="" src="/images//7/76/Fithigeani.gif" width="400" height="300" border="0"/ style="margin-right: 10px;">
<table style="background-color:#ffffff;filter:alpha(opacity= 20);-moz-opacity: 0.5;opacity: 0.5;"
border="1" align="center" width="90%" height="1045" hspace="3" vspace="3" bordercolor="#666666">
<caption align="left">Material</caption>
<tr>
<th align="center" valign="middle" width="200" height="5">Structural formula</th>
<th align="center" valign="middle" width="200" height="5">Name</th>
<th align="center" valign="middle" width="200" height="5">Manufacturer</th>
<th align="center" valign="middle" width="100" height="5">Grade</th>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/2/2c/1.png.gif"/  width="160" height="100" border=""/></td>
<td align="center" valign="middle" width="200" height="130">Sulfo-NHS</td>
<td align="center" valign="middle" width="200" height="130">DOUJINDO</td>
<td align="center" valign="middle" width="100" height="130">200mg
</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/3/3b/FIT%EF%BC%92.png.gif" width="180" height="90" border=""/></td>
<td align="center" valign="middle" width="200" height="130">Tetraethyl orthosilicate<br>
(Tetraethoxysilane; TEOS)
</td>
<td align="center" valign="middle" width="200" height="130">Wako</td>
<td align="center" valign="middle" width="100" height="130">500mg</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/9/98/FIT%EF%BC%93.png.gif" width="180" height="90" border="" /></td>
<td align="center" valign="middle" width="200" height="50">3-aminopropyltriethoxysilane</td>
    <td align="center" valign="middle" width="200" height="50">Wako</td>
    <td align="center" valign="middle" width="100" height="50">25g</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/1/1f/FIT%EF%BC%94.png.png" width="230" height="120" border=""/></td>
<td align="center" valign="middle" width="200" height="130">1 - (3-dimethylaminopropyl) carbodiimide hydrochloride</td>
<td align="center" valign="middle" width="200" height="130">TGI</td>
<td align="center" valign="middle" width="100" height="130">5g</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/c/cf/FIT%E3%80%80%EF%BC%95.gif" width="150" height="100" border=""/></td>
<td align="center" valign="middle" width="200" height="130">N-N-dimethylformamide</td>
<td align="center" valign="middle" width="200" height="130">TGI</td>
<td align="center" valign="middle" width="100" height="130">200mg</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/0/0a/FIT_6.gif" width="145" height="90" border=""/></td>
<td align="center" valign="middle" width="200" height="130">Succinic anhydride</td>
<td align="center" valign="middle" width="200" height="130">Wako</td>
<td align="center" valign="middle" width="100" height="130">25g</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/7/73/FIT_7.gif" width="200" height="70" border=""/></td>
<td align="center" valign="middle" width="200" height="130">Cetyltrimethylammonium bromide</td>
<td align="center" valign="middle" width="200" height="130">Wako</td>
<td align="center" valign="middle" width="100" height="130">25g</td>
</tr>
<tr>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/c/c2/FIT_8.gif" width="180" height="90" border=""/></td>
<td align="center" valign="middle" width="200" height="130">MES
</td><td align="center" valign="middle" width="200" height="130">DOUJINDO
</td><td align="center" valign="middle" width="100" height="130">25g
</td>
</tr>
</table>
 
<br><br><br>
 
<table border="1" rules="rows" style="background-color:#ffffff;filter:alpha(opacity= 20);-moz-opacity: 0.5;opacity: 0.5;"
align="center" width="80%" height="200" hspace="3" vspace="3" bordercolor="#666666">
  <caption align="left">Table 3</caption>
  <tr>
  <th>Fluorescent molecule</th><th>Place</th><th>Excitation wavelength</th><th>Maximum fluorescence wavelength</th>
  </tr>
  <tr align="center" valign="middle">
  <td>TAMRA(red)</td><td>Sword</td><td>550nm</td><td>580nm</td>
  </tr>
  <tr align="center" valign="middle">
  <td>FITC(green)</td><td>Barrel</td><td>498nm</td><td>522nm</td>
  </tr>
</table>
 
<br><br><br>
 
<table style="background-color:#ffffff;filter:alpha(opacity= 20);-moz-opacity: 0.5;opacity: 0.5;" border="1" align="center" width="90%" height="440" hspace="3" vspace="3" bordercolor="#666666">
<caption align="left">Apparatus</caption>
<tr>
<th align="center" valign="middle" width="100" height="50">Device name</th>
<th align="center" valign="middle" width="100" height="50">Manufacturer
Model number
</th>
<th align="center" valign="middle" width="200" height="50">Measurement condition </th>
<th align="center" valign="middle" width="200" height="50">Photograph</th>
        <th align="center" valign="middle" width="200" height="50">Explanations of equipment</th>
</tr>
<tr>
<td align="center" valign="middle" width="100" height="130">Spectrophotometer</td>
<td align="center" valign="middle" width="100" height="130">HITACHI F-2500</td>
<td align="center" valign="middle" width="200" height="130">WL Range:
450nm~650nm
Scanning Speed:
60nm/min
  </td>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/6/6d/FIT_9.png" width="150" height="130" border=""/>  </td>
　　　　　<td align="center" valign="middle" width="200" height="130">This device can see the concentration of the solution, the material characteristics, the structure of the molecule.  </td>
</tr>
<tr>
<td align="center" valign="middle" width="100" height="130">SEM</td>
<td align="center" valign="middle" width="100" height="130">KEYENC VE-7800</td>
<td align="center" valign="middle" width="200" height="130">WL Range:
450nm~650nm
Scanning Speed:
60nm/min
  </td>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/9/9f/FIT_10.png" width="150" height="130" border="">  </td>
　　　　　<td align="center" valign="middle" width="200" height="130">It is possible to significantly change the magnification easily. In addition, photography is possible.  </td>
</tr>
<tr>
<td align="center" valign="middle" width="100" height="130">Confocal laser scanning microscope
(CLSM)
</td>
<td align="center" valign="middle" width="100" height="130">Nikon ECLIPSE-Ti</td>
<td align="center" valign="middle" width="200" height="130">Ar-Laser (488nm)
Garvano mirror scanner
  </td>
<td align="center" valign="middle" width="200" height="130"><img alt="" src="/images/8/8e/FIT_11.png" width="150" height="130" border=""></td>
　　　　　<td align="center" valign="middle" width="200" height="130">It is possible to obtain a high resolution image in focus in the field of view for the entire sample surface with irregularities.
Three-dimensional shape measurement non-destructive, non-contact is possible
  </td>
</tr>
</table>
 
 
 
</center>
</center>
<br>
<hr />


<br><br><br>
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<hr>
</div>
</body>
</body>

Latest revision as of 17:43, 25 October 2014

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  • <a href="http://openwetware.org/wiki/Biomod/2014/Fukuoka#home">Home</a>
  • <a href="fit_Introduction.html#pro">Projects</a>
    • <a href="fit_Introduction.html#back">background & Motivation</a>
    • <a href="fit_Introduction.html#goal">Goals</a>
  • <a href="fit_Approach and Goals.html#des">Design</a>
    • <a href="fit_Approach and Goals.html#ear">Early Design</a>
    • <a href="fit_Approach and Goals.html#fin">Final Design</a>
  • <a href="fit_Method.html#met">Method</a>
    • <a href="fit_Method.html#a">Barrel particle</a>
    • <a href="fit_Method.html#b">DNA modified</a>
    • <a href="fit_Method.html#c">樽と人形の結合</a>
      • 　　　
    • <a href="fit_Method.html#d">樽人形剣</a>
      • 　　　
    • <a href="fit_Method.html#e">Material</a>
  • <a href="fit_Results and Discussion.html#">Result and Discassions</a>
    • <a href="fit_Results and Discussion.html#a">Barrel particle and Doll particle</a>
    • <a href="fit_Results and Discussion.html#b">DNA modified</a>
    • <a href="fit_Results and Discussion.html#c">樽と人形の結合</a>
    • <a href="fit_Results and Discussion.html#d">樽人形剣</a>
      • 　　　
    • <a href="fit_Results and Discussion.html#e">Conclusion</a>
  • <a href="fit_Member.html#team">Team</a>
    • <a href="fit_Member.html#men">Menber</a>
    • <a href="fit_Member.html#spo">Sponsor</a>



Abstract

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Material
Structural formula Name Manufacturer Grade
<img alt="" src="/images/2/2c/1.png.gif"/ width="160" height="100" border=""/> Sulfo-NHS DOUJINDO 200mg
<img alt="" src="/images/3/3b/FIT%EF%BC%92.png.gif" width="180" height="90" border=""/> Tetraethyl orthosilicate

(Tetraethoxysilane; TEOS)

Wako 500mg
<img alt="" src="/images/9/98/FIT%EF%BC%93.png.gif" width="180" height="90" border="" /> 3-aminopropyltriethoxysilane Wako 25g
<img alt="" src="/images/1/1f/FIT%EF%BC%94.png.png" width="230" height="120" border=""/> 1 - (3-dimethylaminopropyl) carbodiimide hydrochloride TGI 5g
<img alt="" src="/images/c/cf/FIT%E3%80%80%EF%BC%95.gif" width="150" height="100" border=""/> N-N-dimethylformamide TGI 200mg
<img alt="" src="/images/0/0a/FIT_6.gif" width="145" height="90" border=""/> Succinic anhydride Wako 25g
<img alt="" src="/images/7/73/FIT_7.gif" width="200" height="70" border=""/> Cetyltrimethylammonium bromide Wako 25g
<img alt="" src="/images/c/c2/FIT_8.gif" width="180" height="90" border=""/> MES DOUJINDO 25g




Table 3
Fluorescent moleculePlaceExcitation wavelengthMaximum fluorescence wavelength
TAMRA(red)Sword550nm580nm
FITC(green)Barrel498nm522nm




　　　　　 　　　　　 　　　　　
Apparatus
Device name Manufacturer

Model number

Measurement condition Photograph Explanations of equipment
Spectrophotometer HITACHI F-2500 WL Range:

450nm~650nm Scanning Speed: 60nm/min

<img alt="" src="/images/6/6d/FIT_9.png" width="150" height="130" border=""/> This device can see the concentration of the solution, the material characteristics, the structure of the molecule.
SEM KEYENC VE-7800 WL Range:

450nm~650nm Scanning Speed: 60nm/min

<img alt="" src="/images/9/9f/FIT_10.png" width="150" height="130" border=""> It is possible to significantly change the magnification easily. In addition, photography is possible.
Confocal laser scanning microscope

(CLSM)

Nikon ECLIPSE-Ti Ar-Laser (488nm)

Garvano mirror scanner

<img alt="" src="/images/8/8e/FIT_11.png" width="150" height="130" border="">It is possible to obtain a high resolution image in focus in the field of view for the entire sample surface with irregularities.

Three-dimensional shape measurement non-destructive, non-contact is possible






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