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Conjugation condition
<b>Conjugation condition</b>




Single strand DNA to folic acid/carbon nanodots/chlorambrucil Ratio  
Single strand DNA to folic acid/carbon nanodots/chlorambrucil Ratio  
1:1
1:1
RTP
RTP
Mixture was vortex for 4 hrs
Mixture was vortex for 4 hrs


In-Complete route
In-Complete route
Folic acid and chlorambrucil were first conjugated and folded, carbon nanodots were added afterwards.
Folic acid and chlorambrucil were first conjugated and folded, carbon nanodots were added afterwards.


Mass-spectrometry
<h4>Mass-spectrometry</h4>
[[Image:expresultSection1_1.jpg|190px]]
 
[[Image:expresultSection1_2.jpg|190px]]
 
[[Image:expresultSection1_3.jpg|190px]]
 
For MS-single stranded DNA A2 , a minor peak at 10120 shows the conjugation of A2 and chlorambrucil.
 
For MS-single stranded DNA A3 , a minor peak at 7043 shows the conjugation of A3 and folic acid.
 
For MS-single stranded DNA A6 , a peak at 11171 shows the conjugation of A6 and carbon nanodots.
 
 
 
<h4>DNA extraction after folding of DNA origami</h4>
Gel photo :
 
[[Image:expresultSection1_4.jpg|190px]]<br>
 
<h4>Nanodrop Reading</h4>
DNA concentration : 0.8 ng/ul
 
260/280 : 1.34


260/230 : 0.86


DNA extraction after folding of DNA origami


Confocal Microscopy
 
 
<h4>Confocal Microscopy</h4>
Hep G2 Treatment
 
[[Image:expresultSection1_5.jpg|190px]][[Image:expresultSection1_6.jpg|190px]][[Image:expresultSection1_7.jpg|190px]]
 
 
Hep G2 Control
 
[[Image:expresultSection1_8.jpg|190px]][[Image:expresultSection1_9.jpg|190px]][[Image:expresultSection1_10.jpg|190px]]
 
Excitation : 405nm
 
Emission : 460 – 560nm


==2nd Trial==
==2nd Trial==


Mass-spectrometry
<b>Conjugation condition</b>
 
Single strand DNA to folic acid/carbon nanodots/chlorambrucil Ratio
 
1:1
 
RTP
 
Mixture was vortex for 4 hrs
 
 
In-Complete route
 
Folic acid and chlorambrucil were first conjugated and folded, carbon nanodots were added afterwards.
 
 
<h4>Mass-spectrometry</h4>
 
[[Image:expresultSection2_1.jpg|190px]]
 
[[Image:expresultSection2_2.jpg|190px]]
 
For MS-single stranded DNA A2 , a minor peak at 10120 shows the conjugation of A2 and chlorambrucil
 
For MS-single stranded DNA A3 , a minor peak at 7043 shows the conjugation of A3 and folic acid
 
 
 
<h4>DNA extraction after folding of DNA origami</h4>
 
Gel photo :
 
[[Image:expresultSection2_3.jpg|190px]]
 
 
<h4>Nanodrop Reading</h4>
 
DNA concentration : 2.3 ng/ul
 
260/280 : 1.28
 
260/230 : 0.52
 
<h4>Confocal Microscopy</h4>
Hep G2 Treatment
 
[[Image:expresultSection2_4.jpg|190px]][[Image:expresultSection2_5.jpg|190px]][[Image:expresultSection2_6.jpg|190px]]


Hep G2 Control
[[Image:expresultSection2_7.jpg|190px]][[Image:expresultSection2_8.jpg|190px]][[Image:expresultSection2_9.jpg|190px]]
Excitation : 405nm
Emission : 460 – 560nm


==3rd Trial==
==3rd Trial==
<b>Conjugation condition</b>
Single strand DNA to folic acid/carbon nanodots/chlorambrucil Ratio
1:10
37oC
Mixture was vortex for 4 hrs
Complete route
All substituent was conjugated and folded simultaneously
<h4>Mass-spectrometry</h4>
[[Image:expresultSection3_1.jpg|190px]]
[[Image:expresultSection3_2.jpg|190px]]
For MS-single stranded DNA A2 , a peak at 10193 shows the conjugation of A2 and chlorambrucil
For MS-single stranded DNA A3 , a peak at 7043 shows the conjugation of A3 and folic acid
<h4>DNA extraction after folding of DNA origami</h4>
Gel photo :
[[Image:expresultSection3_3.jpg|190px]]
<h4>Nanodrop Reading</h4>
DNA concentration : 5.5 ng/ul
260/280 : 2.65
260/230 : 1.12
<h4>Confocal Microscopy</h4>
Hep G2 Treatment
[[Image:expresultSection3_4.jpg|190px]][[Image:expresultSection3_5.jpg|190px]][[Image:expresultSection3_6.jpg|190px]]
Hep G2 Control
[[Image:expresultSection3_7.jpg|190px]][[Image:expresultSection3_8.jpg|190px]][[Image:expresultSection3_9.jpg|190px]]
HK-1 Treatment
[[Image:expresultSection3_10.jpg|190px]][[Image:expresultSection3_11.jpg|190px]][[Image:expresultSection3_12.jpg|190px]]
HK-1 Control
[[Image:expresultSection3_13.jpg|190px]][[Image:expresultSection3_14.jpg|190px]][[Image:expresultSection3_15.jpg|190px]]
Excitation : 405nm
Emission : 460 – 560nm
<h4>Emission Spectrum</h4>
Hep G2                                           HK-1
[[Image:expresultSection3_16.jpg|190px]][[Image:expresultSection3_17.jpg|190px]]
<h4>AFM photo</h4>
[[Image:expresultSection3_18.jpg|190px]][[Image:expresultSection3_19.jpg|190px]]
[[Image:expresultSection3_20.jpg|190px]][[Image:expresultSection3_21.jpg|190px]]

Revision as of 09:31, 23 October 2014

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1st Trial

Conjugation condition


Single strand DNA to folic acid/carbon nanodots/chlorambrucil Ratio

1:1

RTP

Mixture was vortex for 4 hrs


In-Complete route

Folic acid and chlorambrucil were first conjugated and folded, carbon nanodots were added afterwards.

Mass-spectrometry

For MS-single stranded DNA A2 , a minor peak at 10120 shows the conjugation of A2 and chlorambrucil.

For MS-single stranded DNA A3 , a minor peak at 7043 shows the conjugation of A3 and folic acid.

For MS-single stranded DNA A6 , a peak at 11171 shows the conjugation of A6 and carbon nanodots.


DNA extraction after folding of DNA origami

Gel photo :


Nanodrop Reading

DNA concentration : 0.8 ng/ul

260/280 : 1.34

260/230 : 0.86



Confocal Microscopy

Hep G2 Treatment


Hep G2 Control

Excitation : 405nm

Emission : 460 – 560nm

2nd Trial

Conjugation condition

Single strand DNA to folic acid/carbon nanodots/chlorambrucil Ratio

1:1

RTP

Mixture was vortex for 4 hrs


In-Complete route

Folic acid and chlorambrucil were first conjugated and folded, carbon nanodots were added afterwards.


Mass-spectrometry

For MS-single stranded DNA A2 , a minor peak at 10120 shows the conjugation of A2 and chlorambrucil

For MS-single stranded DNA A3 , a minor peak at 7043 shows the conjugation of A3 and folic acid


DNA extraction after folding of DNA origami

Gel photo :


Nanodrop Reading

DNA concentration : 2.3 ng/ul

260/280 : 1.28

260/230 : 0.52

Confocal Microscopy

Hep G2 Treatment


Hep G2 Control

Excitation : 405nm

Emission : 460 – 560nm

3rd Trial

Conjugation condition

Single strand DNA to folic acid/carbon nanodots/chlorambrucil Ratio

1:10

37oC

Mixture was vortex for 4 hrs


Complete route

All substituent was conjugated and folded simultaneously


Mass-spectrometry

For MS-single stranded DNA A2 , a peak at 10193 shows the conjugation of A2 and chlorambrucil

For MS-single stranded DNA A3 , a peak at 7043 shows the conjugation of A3 and folic acid



DNA extraction after folding of DNA origami

Gel photo :


Nanodrop Reading

DNA concentration : 5.5 ng/ul

260/280 : 2.65

260/230 : 1.12


Confocal Microscopy

Hep G2 Treatment


Hep G2 Control

HK-1 Treatment


HK-1 Control

Excitation : 405nm

Emission : 460 – 560nm


Emission Spectrum

Hep G2 HK-1

AFM photo

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