Biomod/2011/Tianjin:Results: Difference between revisions

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=Results=
=Results=
==1==
=='''AFM'''==
[[Image:Biomod tianjin results 1.png|center|650px|thumb|Fig 1.AFM analysis of MreB-RFP cell-free aggregation with SWNTs, indicating that SWNTs serve as scaffolds in the spiral winding of fusion proteins in vitro.
[[Image:Biomod tianjin results 1.png|center|650px|thumb|Fig 1.AFM analysis of MreB-RFP cell-free aggregation with SWNTs, indicating that SWNTs serve as scaffolds in the spiral winding of fusion proteins in vitro.
*a). AFM image of fusion protein MreB-RFP without SWNT scaffold, protein aggregated in disorder and only discrete spherical particles were formed.
a). AFM image of fusion protein MreB-RFP without SWNT scaffold, protein aggregated in disorder and only discrete spherical particles were formed.
*b). AFM image of fusion protein MreB-RFP at the presence of SWNT, protein aggregated
b). AFM image of fusion protein MreB-RFP at the presence of SWNT, protein aggregated
and wound spirally onto the surface of nanotube.  
and wound spirally onto the surface of nanotube.  
  ]]
  ]]


==2==
=='''SEM'''==
[[Image:Biomod tianjin results 24.png|center|650px|thumb|Fig 2. SEM analysis of MreB-RFP cell-free aggregation with SWNTs.
[[Image:Biomod tianjin results 24.png|center|650px|thumb|Fig 2. SEM analysis of MreB-RFP cell-free aggregation with SWNTs.
*a). SEM image of SWNT, the sidewall of nanotube is smooth
a). SEM image of SWNT, the sidewall of nanotube is smooth
without any absorption or aggregation.
without any absorption or aggregation.
*b). SEM image of MreB-RFP without scaffold, protein aggregated
b). SEM image of MreB-RFP without scaffold, protein aggregated
in disorder and only discrete spherical particles were formed.
in disorder and only discrete spherical particles were formed.
*c). SEM image of fusion protein at the presence of SWNT, protein
c). SEM image of fusion protein at the presence of SWNT, protein
  aggregated and wound spirally onto the surface of nanotube.  
  aggregated and wound spirally onto the surface of nanotube.  
  ]]
  ]]


==3==
=='''TEM'''==
[[Image:Biomod tianjin results 3.png|center|650px|thumb|Fig 3. TEM analysis of MreB-RFP cell-free aggregation with SWNTs.
[[Image:Biomod tianjin results 3.png|center|650px|thumb|Fig 3. TEM analysis of MreB-RFP cell-free aggregation with SWNTs.
*a). TEM image of SWNT, the sidewall of nanotube is smooth
a). TEM image of SWNT, the sidewall of nanotube is smooth
without any absorption or aggregation.
without any absorption or aggregation.
*b). TEM image of MreB-RFP without scaffold, protein aggregated
b). TEM image of MreB-RFP without scaffold, protein aggregated
in disorder and only discrete spherical particles were formed.
in disorder and only discrete spherical particles were formed.
*c). TEM image of fusion protein without SWNT scaffold, protein
c). TEM image of fusion protein without SWNT scaffold, protein
  aggregated and wound spirally onto the surface of SWNT.  
  aggregated and wound spirally onto the surface of SWNT.  
]]
]]


==4==
=='''FM'''==
[[Image:Biomod tianjin results 4.png|center|500px|thumb|Fig 4. Fluorescence micrograph of fusion protein MreB combined with SWNT. Red fluorescence can be observed, indicating the activity of fusion protein were reserved. ]]
[[Image:Biomod tianjin results 4.png|center|500px|thumb|Fig 4. Fluorescence micrograph of fusion protein MreB combined with SWNT. Red fluorescence can be observed, indicating the activity of fusion protein were reserved. ]]




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Revision as of 12:00, 31 October 2011


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Results

AFM

Fig 1.AFM analysis of MreB-RFP cell-free aggregation with SWNTs, indicating that SWNTs serve as scaffolds in the spiral winding of fusion proteins in vitro. a). AFM image of fusion protein MreB-RFP without SWNT scaffold, protein aggregated in disorder and only discrete spherical particles were formed. b). AFM image of fusion protein MreB-RFP at the presence of SWNT, protein aggregated and wound spirally onto the surface of nanotube.

SEM

Fig 2. SEM analysis of MreB-RFP cell-free aggregation with SWNTs. a). SEM image of SWNT, the sidewall of nanotube is smooth without any absorption or aggregation. b). SEM image of MreB-RFP without scaffold, protein aggregated in disorder and only discrete spherical particles were formed. c). SEM image of fusion protein at the presence of SWNT, protein aggregated and wound spirally onto the surface of nanotube.

TEM

Fig 3. TEM analysis of MreB-RFP cell-free aggregation with SWNTs. a). TEM image of SWNT, the sidewall of nanotube is smooth without any absorption or aggregation. b). TEM image of MreB-RFP without scaffold, protein aggregated in disorder and only discrete spherical particles were formed. c). TEM image of fusion protein without SWNT scaffold, protein aggregated and wound spirally onto the surface of SWNT.

FM

Fig 4. Fluorescence micrograph of fusion protein MreB combined with SWNT. Red fluorescence can be observed, indicating the activity of fusion protein were reserved.


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