Biomod/2011/Tianjin:Project: Difference between revisions
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===CFS and Microfluidics=== | ===CFS and Microfluidics=== | ||
*To mimic the natural cell more faithfully, cell-free expression systems have been combined and encapsulated into double emulsion droplets, which is produced by microfluidcs technology. Water in oil in water (W/O/W) droplets are created by using a hybrid Lab-chip devices comprising a hydrophobic network (supporting a continuous oil phase), interfaced with a hydrophilic network (supporting an aqueous phase). | *To mimic the natural cell more faithfully, cell-free expression systems have been combined and encapsulated into double emulsion droplets, which is produced by microfluidcs technology. Water in oil in water (W/O/W) droplets are created by using a hybrid Lab-chip devices comprising a hydrophobic network (supporting a continuous oil phase), interfaced with a hydrophilic network (supporting an aqueous phase). | ||
*These microdroplets are of comparable scales to natural cells and could serve as a platform where biochemical reactions can take place, for example, the cell-free expression of water-soluble protein Green Fluorescent Protein (GFP). Using mineral oil as the oil phase would provide a robust and stable membrane, and the hydrophobic interactions generated within this membrane environment produce driving forces which account for the distribution of different intracellular proteins, and mimic the natural cell membrane to provide a non-polar environment which separates the extracellular aqueous environment from the cell’s internal aqueous environment. | *These microdroplets are of comparable scales to natural cells and could serve as a platform where biochemical reactions can take place, for example, the cell-free expression of water-soluble protein Green Fluorescent Protein (GFP). Using mineral oil as the oil phase would provide a robust and stable membrane, and the hydrophobic interactions generated within this membrane environment produce driving forces which account for the distribution of different intracellular proteins, and mimic the natural cell membrane to provide a non-polar environment which separates the extracellular aqueous environment from the cell’s internal aqueous environment. | ||
===CFS and SWNTs (Single-Walled Carbon Nanotubes)=== | ===CFS and SWNTs (Single-Walled Carbon Nanotubes)=== |