Biomod/2011/TUM/TNT/LabbookA/list TEM pictures

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<tr bgcolor="#f5f5f5"><td valign="top">B3</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/7/7c/20111013_theU_BM2_2.27uM_EtBr_B3.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 13th</td><td valign="top">good stain and structure concentration, 36 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B3</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/7/7c/20111013_theU_BM2_2.27uM_EtBr_B3.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 13th</td><td valign="top">good stain and structure concentration, 36 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B4</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/b/b5/20111014_theU_BM2_2.27uM_EtBr_B4.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 14th</td><td valign="top">good stain but slightly to low structure concentration, 35 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B4</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/b/b5/20111014_theU_BM2_2.27uM_EtBr_B4.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 14th</td><td valign="top">good stain but slightly to low structure concentration, 35 images</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">B5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">4</td><td valign="top">5</td></tr>
+
<tr bgcolor="#f5f5f5"><td valign="top">B5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top"></td><td valign="top"></td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B6</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/0/0a/20111021_theU_BM2_2B6_control.png">BM2 without any DNA binders, 1:25</a></td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 66  images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B6</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/0/0a/20111021_theU_BM2_2B6_control.png">BM2 without any DNA binders, 1:25</a></td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 66  images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B7</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/9/9a/20111021_theU_BM2_2B7_control.png">BM2 without any DNA binders, 1:25</a></td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 80 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">B7</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/9/9a/20111021_theU_BM2_2B7_control.png">BM2 without any DNA binders, 1:25</a></td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 80 images</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">C1</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/3/33/20111014_theU_BM2_0.69uM_EtBr_C1.png">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</a></td><td valign="top">oct. 14th</td><td valign="top">good stain but dilution slightly to low, 32 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">C1</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/3/33/20111014_theU_BM2_0.69uM_EtBr_C1.png">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</a></td><td valign="top">oct. 14th</td><td valign="top">good stain but dilution slightly to low, 32 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">C2</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/4/44/20111014_theU_BM2_0.69uM_EtBr_C2.png">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</a></td><td valign="top">oct. 14th</td><td valign="top">good stain but dilution slightly to low, 31 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">C2</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/4/44/20111014_theU_BM2_0.69uM_EtBr_C2.png">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</a></td><td valign="top">oct. 14th</td><td valign="top">good stain but dilution slightly to low, 31 images</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">C3</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr>
+
<tr bgcolor="#f5f5f5"><td valign="top">C3</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top"></td><td valign="top"></td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">C4</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr>
+
<tr bgcolor="#f5f5f5"><td valign="top">C4</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top"></td><td valign="top"></td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">C5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr>
+
<tr bgcolor="#f5f5f5"><td valign="top">C5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top"></td><td valign="top"></td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">C6</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.pnghttp://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.png">BM24 15_65 dilution 1:20 without any DNA binders</a></td><td valign="top">oct. 21th</td><td valign="top">stain was bad and to high dilution, 17 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">C6</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.pnghttp://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.png">BM24 15_65 dilution 1:20 without any DNA binders</a></td><td valign="top">oct. 21th</td><td valign="top">stain was bad and to high dilution, 17 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">C7</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/8/89/20111021_theU_BM24_2C7_2DH3ML.png">BM24 2DH3ML dilution 1:20 without any DNA binders </a></td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high, 70 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">C7</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/8/89/20111021_theU_BM24_2C7_2DH3ML.png">BM24 2DH3ML dilution 1:20 without any DNA binders </a></td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high, 70 images</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">D5</td><td valign="top">oct. 14th</td><td valign="top"><a href="http://openwetware.org/images/1/18/20111020_theU_2d5_144nM_dapi.png">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 20th</td><td valign="top">stain and dilution was good, cluster, 61 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">D5</td><td valign="top">oct. 14th</td><td valign="top"><a href="http://openwetware.org/images/1/18/20111020_theU_2d5_144nM_dapi.png">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 20th</td><td valign="top">stain and dilution was good, cluster, 61 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">D6</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/3/32/20111027_theU_BM24_2D6_control.png">BM24 (15_65), 1:20 diluted with FOB20</a></td><td valign="top">oct. 27th</td><td valign="top">good stain but slightly to high dilution, 22 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">D6</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/3/32/20111027_theU_BM24_2D6_control.png">BM24 (15_65), 1:20 diluted with FOB20</a></td><td valign="top">oct. 27th</td><td valign="top">good stain but slightly to high dilution, 22 images</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">D7</td><td valign="top">oct. 27th</td><td valign="top">lost the grid before sample preparation</td><td valign="top">4</td><td valign="top">5</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">D7</td><td valign="top">oct. 27th</td><td valign="top">lost the grid before sample preparation</td><td valign="top"></td><td valign="top"></td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">D8</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/9/9d/20111027_theU_BM24_2D8_control024.png">BM24 (15_65) 1:10 without any DNA binders</a></td><td valign="top">oct. 27th</td><td valign="top">low sample concentration, 24 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">D8</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/9/9d/20111027_theU_BM24_2D8_control024.png">BM24 (15_65) 1:10 without any DNA binders</a></td><td valign="top">oct. 27th</td><td valign="top">low sample concentration, 24 images</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">D9</td><td valign="top">oct. 27th</td><td valign="top"><a href="">BM24 (15_65) 1:10 without any DNA binders</a></td><td valign="top">4</td><td valign="top">,stain was good but dilution slightly to low</td></tr>
+
<tr bgcolor="#f5f5f5"><td valign="top">D9</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65) 1:10 without any DNA binders</td><td valign="top"></td><td valign="top">,stain was good but dilution slightly to low</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">D10</td><td valign="top">oct. 28st</td><td valign="top"><a href="http://openwetware.org/images/d/d1/20111028_theU_BM24_2D10_control.png">BM24 (15_65) 1:5 without any DNA binders</a></td><td valign="top">oct. 28st</td><td valign="top"> better sample concentration than D6 and D8, 60 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">D10</td><td valign="top">oct. 28st</td><td valign="top"><a href="http://openwetware.org/images/d/d1/20111028_theU_BM24_2D10_control.png">BM24 (15_65) 1:5 without any DNA binders</a></td><td valign="top">oct. 28st</td><td valign="top"> better sample concentration than D6 and D8, 60 images</td></tr>
<tr><td valign="top"></td></tr>
<tr><td valign="top"></td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">E1</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</td><td valign="top">oct. 26th</td><td valign="top">good dilution, bad staining, 49 images</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">E1</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/1/18/20111101_theU_Bm2_2e1_2_4um_etbr.png">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 26th</td><td valign="top">good dilution, bad staining, 49 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">E2</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/a/a7/20111026_theU_BM2_2E2_2.4uM_EtBr049.png">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 26th</td><td valign="top">good dilution but strong background, 50 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">E2</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/a/a7/20111026_theU_BM2_2E2_2.4uM_EtBr049.png">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 26th</td><td valign="top">good dilution but strong background, 50 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">E3</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/f/f9/20111026_theU_BM2_2E3_0.74uM_EtBr059.png">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)</a><td valign="top">oct. 26th</td><td valign="top">fine grid, 60 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">E3</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/f/f9/20111026_theU_BM2_2E3_0.74uM_EtBr059.png">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)</a><td valign="top">oct. 26th</td><td valign="top">fine grid, 60 images</td></tr>

Revision as of 00:49, 3 November 2011

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TEM


All dilutions of the structures were made with FOB20. For the calculation of the intercalator concentration read more in the corresponding labbook entry. Due to a limitation in webspace we only uploaded a selection of 6 pictures for each grid.

Contents

BIOMOD1 Grid Box

grid number   prepared   annotations   observed   annotation
B2sep. 16thBM2 1:5 control without DNA-binderssep. 21thfine grid
B5sep. 16thBM2 1:5 with 100 µM sperminesep. 21thstructures accumulated
C2sep. 16thBM2 1:5 with 25 µM ethidium bromidesep. 21thmassive deformation of the structures
C3sep. 16thBM2 1:5 with 75 µM ethidium bromidesep. 21thbroken grid
C4sep. 16thBM2 1:5 with 250 µM ethidium bromidesep. 21thdestroyed structures, not enough structures

BIOMOD2 Grid Box

grid number   prepared   annotations   observed   annotation
A1oct. 5thBM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)oct. 7thgood stain, slightly to low dilution, 62 images
A2oct. 5thBM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)oct. 7thgood stain, slightly to low dilution, 63 images
A3oct. 5thBM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)oct. 7thgood stain, slightly to low dilution, 60 images
A4oct. 5thBM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)oct. 7thgood stain, slightly to low dilution, 64 images
A5oct. 5thBM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)oct. 7thgood stain, slightly to low dilution, 63 images
A6oct. 20thBM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)oct. 28stno origamis on the grid, no images
A7oct. 20thBM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)oct. 27thno origamis on the grid, no images
A8oct. 20thBM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)oct. 28thno origamis on the grid, no images
A9oct. 20thBM2 1:25 without any DNA bindersoct. 21thgood stain and dilution, 40 images
A10oct. 20thBM2 1:25 without any DNA bindersoct. 21thgood stain and dilution, 60 images
B1oct. 5thBM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)oct. 12thgrid was fine, 60 images
B2oct. 10thBM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)oct. 12thtoo high dilution, 25 images
B3oct. 10thBM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)oct. 13thgood stain and structure concentration, 36 images
B4oct. 10thBM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)oct. 14thgood stain but slightly to low structure concentration, 35 images
B5oct. 10thBM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)
B6oct. 20thBM2 without any DNA binders, 1:25oct. 21thgood stain and dilution, 66 images
B7oct. 20thBM2 without any DNA binders, 1:25oct. 21thgood stain and dilution, 80 images
B8oct. 20thBM24 15_65 1:25 without any DNA bindersoct. 20thbad stain and to high dilution
B9oct. 20thBM24 2DH3ML 1:25 without any DNA binders oct. 20thbad stain, no structures on the grid, 3 images
B10oct. 21thBM24 15_65 1:20 without any DNA bindersoct. 21thgood stain but slightly to high dilution, 61 images
C1oct. 10thBM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)oct. 14thgood stain but dilution slightly to low, 32 images
C2oct. 10thBM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)oct. 14thgood stain but dilution slightly to low, 31 images
C3oct. 10thBM2 1:40 without any DNA binders
C4oct. 10thBM2 1:40 without any DNA binders
C5oct. 10thBM2 1:40 without any DNA binders
C6oct. 21thBM24 15_65 dilution 1:20 without any DNA bindersoct. 21thstain was bad and to high dilution, 17 images
C7oct. 21thBM24 2DH3ML dilution 1:20 without any DNA binders oct. 21thstain was good but dilution slightly to high, 70 images
C8oct. 21thBM24 2DH3ML 1:20 without any DNA bindersoct. 21thstain was good but dilution slightly to high
C9oct. 27thBM2 1:25 without any DNA bindersoct. 27good stain but to high dilution, 60 images
C10oct. 27thBM2 1:25 without any DNA bindersoct. 27thfine grid, 60 images
D1oct. 12thBM2 1:25 with 2.4 µM ethidium bromide (corresponding to one molecule every 7bp)oct. 12thgood stain and dilution, 74 images
D2oct. 12thBM2 1:25 with 0.74 µM ethidium bromide (corresponding to one molecule every 21bp)oct. 12thgood stain and dilution, 89 images, chosen for class average
D3oct. 12thBM2 1:25without any DNA binders oct. 12thgood stain and dilution, 50 images
D4oct. 14thBM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp)oct. 20thgood stain and structur concentration but cluster, 62 images
D5oct. 14thBM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)oct. 20thstain and dilution was good, cluster, 61 images
D6oct. 27thBM24 (15_65), 1:20 diluted with FOB20oct. 27thgood stain but slightly to high dilution, 22 images
D7oct. 27thlost the grid before sample preparation
D8oct. 27thBM24 (15_65) 1:10 without any DNA bindersoct. 27thlow sample concentration, 24 images
D9oct. 27thBM24 (15_65) 1:10 without any DNA binders,stain was good but dilution slightly to low
D10oct. 28stBM24 (15_65) 1:5 without any DNA bindersoct. 28st better sample concentration than D6 and D8, 60 images
E1oct. 20thBM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)oct. 26thgood dilution, bad staining, 49 images
E2oct. 20thBM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)oct. 26thgood dilution but strong background, 50 images
E3oct. 20thBM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)oct. 26thfine grid, 60 images
E4oct. 20thBM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)oct. 26thfine grid, 50 images
E5oct. 20thBM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)oct. 26thgood dilution but weak stain, 60 images
E6oct. 28stBM24 (15_65), 1:5oct. 28stfine grid, 413 images
E7oct. 31thBM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)oct. 31thgrid was fine, 112 images
E8oct. 31thBM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)oct. 31thexcellent grid, 80 images
E9nov. 1stBM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)nov. 1stgood grid, 111 images
E10nov. 1stBM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)nov. 1stfine grid, 131 images