Biomod/2011/TUM/TNT/LabbookA/2011/09/01: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Silvia Blank (talk | contribs) |
Silvia Blank (talk | contribs) |
||
| Line 8: | Line 8: | ||
==Real time PCR with MH255_647 and MH256_550 adding ErBr and Spermine== | ==Real time PCR with MH255_647 and MH256_550 adding ErBr and Spermine== | ||
* MH255_647 and MH256_550 are oligonucleotides of 18 nucleotides each, labeled with Atto fluorescent dyes Atto 647 and Atto 550. | * MH255_647 and MH256_550 are oligonucleotides of 18 nucleotides each, labeled with Atto fluorescent dyes Atto 647 and Atto 550. | ||
* The DNA intercalator ethidium bromide and the major groove binder Spermine are added at various concentrations accoring to their | * The DNA intercalator ethidium bromide and the major groove binder Spermine are added at various concentrations accoring to their K<sub>D</sub>-Values. | ||
* The | * The K<sub>D</sub> value of Spermin is 4,8µL and that of ethidium bromide is 12µL. | ||
* The concentrations of Spermin and ethidium bromide are as follows: | * The concentrations of Spermin and ethidium bromide are as follows: | ||
1/19*Kd, 1/9*Kd, 1/4*Kd, 1/2*KD, 1*Kd, 2*Kd, 4*Kd, 9*Kd, 19*Kd. | |||
Revision as of 03:44, 2 September 2011
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Real time PCR with MH255_647 and MH256_550 adding ErBr and Spermine
1/19*Kd, 1/9*Kd, 1/4*Kd, 1/2*KD, 1*Kd, 2*Kd, 4*Kd, 9*Kd, 19*Kd.
| |
