Beta-glucuronidase protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 5: | Line 5: | ||
==Plate Screen== | ==Plate Screen== | ||
===Materials=== | ===Materials=== | ||
*Media of choice | *Media of choice | ||
*X-gluc stock solution ( | *Agar | ||
*X-gluc stock solution (50mg/mL) | |||
===Method=== | ===Method=== | ||
#Prepare your liquid media and add the desired amount of agar (usually 1-2%). | |||
#Autoclave the media for the requisite time. | |||
#Add 1.2μL of X-gluc stock solution for every mL of media (e.g. if making 1L of media add 1.2ml of stock solution) | |||
#If desired, add antibiotic. | |||
#Pour plates. | |||
===Notes=== | ===Notes=== | ||
Revision as of 22:38, 4 July 2011
Introduction
These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols.
Plate Screen
Materials
- Media of choice
- Agar
- X-gluc stock solution (50mg/mL)
Method
- Prepare your liquid media and add the desired amount of agar (usually 1-2%).
- Autoclave the media for the requisite time.
- Add 1.2μL of X-gluc stock solution for every mL of media (e.g. if making 1L of media add 1.2ml of stock solution)
- If desired, add antibiotic.
- Pour plates.
