Calibration
After setting up the black box and putting the fluorimeter inside the black box and turning it on, make sure to get a ruler with centimeters and the phone
cradle. Then put down the cradle in front of the fluorimeter facing its side and place the phone in the cradle to accurately measure the distance
needed.Measure 4 cm from both sides of the cradle in respect of the phone's position to have a close to precise measurement of distance.
Distance between the smart phone cradle and drop = 4 cm
Solutions Used for Calibration
Placing Samples onto the Fluorimeter
[Step one, Set the micropipettor to 120 microliters (μL) and obtain 100μL of the DNA samples. ]
[Step two, Take 100μL of the DNA sample and transfer it to their respectable big Buffer tubes. ]
[Step three, ]
[Step four,]
[Step five, ]
[Step six, ]
[Step seven, ]
[Step eight, ]
[Step nine, ]
Data Analysis
Representative Images of Negative and Positive Samples
Image J Values for All Calibrator Samples
TABLE GOES HERE
Calibration curve
PCR Results Summary
Our positive control PCR result was ____ μg/mL
Our negative control PCR result was ____ μg/mL
Observed results
Patient _____ :
Patient _____ :
Conclusions
Patient _____ :
Patient _____ :
SNP Information & Primer Design
Background: About the Disease SNP
The disease CHD also known as coronary heart disease is a cardiac risk that occurs