BME100 s2015:Group5 9amL4: Difference between revisions
Jacob Licko (talk | contribs) |
|||
Line 93: | Line 93: | ||
'''Q1: What is the function of each component of a PCR reaction''' <br> | '''Q1: What is the function of each component of a PCR reaction''' <br> | ||
Template DNA: This is the sequence of DNA that is intended for amplification. It will undergo extreme heat in order for it to denature. | |||
Primers: A primer is a single strand of DNA that serves as the starting point for DNA synthesis | |||
Taq Polymerase: This is a thermostable enzyme that recombines nucleotides following separation by heat. It creates a new, complimentary strand of DNA through adding new nucleotides to the old ones. | |||
Deoxyribonucleotides (dNTP's): There are the building blocks of DNA: adenine (A), thymine (T), cytosine (C) and guanine (G). These are used by the Taq Polymerase to synthesize the complementary strand of DNA. | |||
'''Q2: What happens to the components (listed above) during each step of thermal cycling?''' <br> | '''Q2: What happens to the components (listed above) during each step of thermal cycling?''' <br> |
Revision as of 17:34, 31 March 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
Research and DevelopmentPCR - The Underlying Technology Q1: What is the function of each component of a PCR reaction Template DNA: This is the sequence of DNA that is intended for amplification. It will undergo extreme heat in order for it to denature. Primers: A primer is a single strand of DNA that serves as the starting point for DNA synthesis Taq Polymerase: This is a thermostable enzyme that recombines nucleotides following separation by heat. It creates a new, complimentary strand of DNA through adding new nucleotides to the old ones. Deoxyribonucleotides (dNTP's): There are the building blocks of DNA: adenine (A), thymine (T), cytosine (C) and guanine (G). These are used by the Taq Polymerase to synthesize the complementary strand of DNA. Q2: What happens to the components (listed above) during each step of thermal cycling? Q3: DNA is made up of four types of molecules called nucleotides, designated as A, T, C and G. Base-pairing, driven by hydrogen bonding, allows base pairs to stick together. Which base anneals to each base listed below?;;;
Q4: During which two steps of thermal cycling does base-pairing occur? Explain your answers.
|