BME100 s2015:Group3 9amL4: Difference between revisions
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'''DNA Sample Set-up Procedure''' | '''DNA Sample Set-up Procedure''' | ||
<!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes in to the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. --> | <!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes in to the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. --> | ||
# | # Record a list of materials, samples and the tube labels. | ||
# | # Cut the strip of empty PCR tubes in half so that you have two strips of four linked tubes. This is necessary to fit all of your tubes into the OpenPCR machine. | ||
# | # Use a black marker to label the sides of the empty tubea. | ||
# Place the tubes in a rack. | |||
# Starting with the empty tube labeled as the positive control, transfer 50 μL of PCR reaction mix into an empty tube. | |||
# Transfer the positive control DNA/ primer mix into the same tube. | |||
# Repeat steps for the negative control, patient 1 and patient 2 samples. | |||
# Close the lids on PCR reaction tubes. | |||
# Place tubes into PCR machine. | |||
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[[Image:OpenPCR program.png|800px|PCR image]] | |||
Latest revision as of 22:45, 31 March 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
OpenPCR program
Research and DevelopmentPCR - The Underlying Technology What is the function of each component of a PCR reaction
What happens to the componenets (listed above) during each step of thermal cycling?
DNA is made up of four types of molecules called nucleotides, designated as A, T, C and G. Base-pairing driven by hydrogen bonding, allows base pairs to stick together. Which base anneals to each base listed below? Adenine(A) : Thymine(T) Thymine(T) : Adenine(A) Cytosine(C): Guanine(G) Guanine(G) : Cytosine(C) During which two steps of thermal cycling does base-pairing occur? Explain your answers. Base-pairing occurs during the extended and final step of the PCR reaction. AT 72°C, the enzyme taq polymerase is at it's optimal temperature and proceeds to attach to primers on template DNA strands. Once attached, the enzyme attains the proper base pairs to create a complementary DNA strand for amplification.
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