BME100 s2015:Group2 9amL4: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 99: | Line 99: | ||
Q2. What happens to the components (listed above) during each step of thermal cycling? | Q2. What happens to the components (listed above) during each step of thermal cycling? | ||
*INITIAL STEP: 95°C for 3 minutes: | *INITIAL STEP: 95°C for 3 minutes: In order for the strand to denature completely, it is thoroughly heated to 95°C. | ||
*Denature at 95°C for 30 seconds: | *Denature at 95°C for 30 seconds: | ||
*Anneal at 57°C for 30 seconds: | *Anneal at 57°C for 30 seconds: | ||
*Extend at 72°C for 30 seconds: | *Extend at 72°C for 30 seconds: |
Revision as of 22:52, 31 March 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
PCR Reaction Sample List
Denature at 95°C for 30 seconds, Anneal at 57°C for 30 seconds, and *Extend at 72°C for 30 seconds
Research and DevelopmentPCR - The Underlying Technology
|