BME100 s2015:Group2 9amL4: Difference between revisions
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Q1. What is the function of each component of a PCR reaction? | |||
*Template DNA: | |||
*Primers: | |||
*Taq Polymerase: | |||
*Deoxyribonucleotides (dNTP’s): | |||
Q2. What happens to the components (listed above) during each step of thermal cycling? | |||
*INITIAL STEP: 95°C for 3 minutes: | |||
*Denature at 95°C for 30 seconds: | |||
*Anneal at 57°C for 30 seconds: | |||
*Extend at 72°C for 30 seconds: | |||
*FINAL STEP: 72°C for 3 minutes: | |||
*FINAL HOLD: 4°C: | |||
Q3. DNA is made up of four types of molecules called nucleotides, designated as A, T, C and G. Base-pairing, driven by hydrogen bonding, allows base pairs to stick together. Which base anneals to each base listed below? | |||
*Adenine (A): | |||
*Thymine (T): | |||
*Cytosine (C): | |||
*Guanine (G): | |||
Q4. During which two steps of thermal cycling does base-pairing occur? Explain your answers. | |||
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Revision as of 20:18, 31 March 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
PCR Reaction Sample List
Denature at 95°C for 30 seconds, Anneal at 57°C for 30 seconds, and *Extend at 72°C for 30 seconds
Research and DevelopmentPCR - The Underlying Technology
Q2. What happens to the components (listed above) during each step of thermal cycling?
*Denature at 95°C for 30 seconds:
Q3. DNA is made up of four types of molecules called nucleotides, designated as A, T, C and G. Base-pairing, driven by hydrogen bonding, allows base pairs to stick together. Which base anneals to each base listed below?
Q4. During which two steps of thermal cycling does base-pairing occur? Explain your answers.
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