BME100 s2015:Group2 12pmL6: Difference between revisions
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The consumables that will be packaged in the kit will be: | The consumables that will be packaged in the kit will be: | ||
* | * PCR reagent mixers | ||
* test tubes to hold samples | |||
* 32 test tubes which are 50 μL of content in each: Mix contains Taq DNA polymerase, MgCl2, and dNTP’s | |||
* DNA/ primer mix, 32 tubes, 50 μL each: All tubes contain the correct reverse and forward primer | |||
* 32 empty PCR tubes | |||
* Pipette tips | |||
* Micropipettor | |||
* SYBR Green I Dye | |||
* Glass Slides For Fluorimeter | |||
For the way in which these consumables will be packaged, they will come in a box which contains each section divided appropriately so that each consumable will be grouped together. The box will have a flip-top lid which moves backwards and has printed labels at the top which identify exactly which consumable is in each compartment. The consumables will be grouped together and allow for the production of thirty two PCR reactions. This means that the contents will be enough to fill the newly designed PCR machine for one full run in which each compartment is entirely filled with an individual PCR tube. The benefit of this is that the new consumables are organized and easy to keep track of that so that the experimenter will never get the components mixed up. The new feature that this group is offering is also the ability to order specific primers in the consumables package so that the PCR can be ran for whatever the consumer needs. Also, the new pipette tips will allow for reuse so that they do not need to be thrown away. When consumers order new consumable packages, there will be an option on whether to include or not include pipette tips. This is a customizable feature to help the consumer and allow them to spend less money in the long run. | |||
A major problem with the consumables in our kit used in this experiment were the amount of plastic disposed. As engineers, sustainability should always be a top priority when conducting an experiment. As a solution to this problem, the plastic tubes used for micro-pipetting should be produced using more durable material and eco-friendly materials. This material will have to be one where it will not absorb the sample or liquid that it is picking up, so as to not contaminate later samples. With more durable and eco-friendly materials, these plastic tubes can then be sanitized and reused multiple times before disposal, thus lessening the amounts of plastic waste. Also, with eco-friendly material, the decomposition of these tubes will not contribute to rapid increase of plastic pollution in the environment. | A major problem with the consumables in our kit used in this experiment were the amount of plastic disposed. As engineers, sustainability should always be a top priority when conducting an experiment. As a solution to this problem, the plastic tubes used for micro-pipetting should be produced using more durable material and eco-friendly materials. This material will have to be one where it will not absorb the sample or liquid that it is picking up, so as to not contaminate later samples. With more durable and eco-friendly materials, these plastic tubes can then be sanitized and reused multiple times before disposal, thus lessening the amounts of plastic waste. Also, with eco-friendly material, the decomposition of these tubes will not contribute to rapid increase of plastic pollution in the environment. |
Revision as of 14:36, 15 April 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||
OUR COMPANY
LAB 6 WRITE-UPBayesian StatisticsOverview of the Original Diagnosis System
What Bayes Statistics Imply about This Diagnostic Approach
The results for calculation 2 where Variable A meant there was a negative test conclusion while variable B showed the negative PCR reaction. Both variable A and B showed the number of patients diagnosed and tested negative produced Bayes values that were slightly above .500 being 2% different from each other, indicating both negative test results correlate with each other through the experiment. Taking the frequency from both variables, where the probability of having a negative PCR reaction given negative test conclusions shows that both variables correlate with each other having a probability of 94%. Inversely, the probability of a negative PCR result given the conclusion being negative gives a probability of 90%. In conclusion, a negative Final Test Conclusion and Negative PCR reaction does in fact correlate with each other. The results for calculation 3 where Variable A meant the total patient that will develop disease while Variable B shows the positive test conclusion. Variable A producing value of 37% and variable B producing a value of 43%. Now the probability of variable B given A, Frequency of positive test conclusion given patient that will develop disease, gives a value of .9 or 90% means that the number of patients that do test positive will have a high probability of developing the disease. Now the probability of variable A given B, total patients that will develop the disease given the probability of positive final test conclusions, gives a value of .77 or 77% means that the patient that will develop the disease later on will test positive during the final test but not completely conclusive. Since the value is not close to 1.00 there were errors in the value or the actual experiment. The results for calculation 4 where Variable A meant the total patients that will not develop the disease while variable B shows the total negative test conclusions. Variable A showed the Bayes value being close to .500 or 56% and Variable B shows a value above .60 or 62%. Taking the frequency for both values where the probability of variable B given A, negative Test Conclusion given Total Patient that will not develop disease, gives a Bayes value close to 1.00 or 94% which shows that yes, patients that test negative will be likely to not develop the disease. Now the probability of variable A given B, total patients that will not develop the disease given the probability of the negative final test conclusion, gives a frequency that is 84%. Meaning that majority of the total patients that do not develop the disease will be more likely to have a negative final test conclusion though not completely conclusive. Computer-Aided DesignTinkerCAD The new design was chosen to address the problem regarding amount of samples which can be tested. The original openPCR device only could fit 16 micro-tubes, which only allowed for 16 tests, and in the application of this course, only allowed for two teams per box. This new design will expand the amount of tests per box, allowing for more tests, the new openPCR design has double the area for microtubes, allowing for double the amount of tubes. The new design will accommodate up to 32 micro-tubes. In order to do this, the group assembled the outside of the Open PCR machine and increased the length and width so that the dimension created a doubling of the inside area. This doubling in area means that double the amount of test tubes will be able to be tested.
Feature 1: Consumables KitThe consumables that will be packaged in the kit will be:
A major problem with the consumables in our kit used in this experiment were the amount of plastic disposed. As engineers, sustainability should always be a top priority when conducting an experiment. As a solution to this problem, the plastic tubes used for micro-pipetting should be produced using more durable material and eco-friendly materials. This material will have to be one where it will not absorb the sample or liquid that it is picking up, so as to not contaminate later samples. With more durable and eco-friendly materials, these plastic tubes can then be sanitized and reused multiple times before disposal, thus lessening the amounts of plastic waste. Also, with eco-friendly material, the decomposition of these tubes will not contribute to rapid increase of plastic pollution in the environment. Feature 2: Hardware - PCR Machine & FluorimeterThe PCR machine and fluorimeter that will be included in the system will remain the same as the original.
The PCR machine will include holder for 32 test tubes instead of 16, so that more samples can be analyzed in lesser time. Also, A camera would have to be implanted to take photos of the samples, hence an amateur cell phone camera does not have to be used. This will lead to better pictures to analyze and more accurate and precise data. Moreover, the photos taken by the camera would be automatically transmitted to a computer that is synced with the PCR machine so that all the results can be automatically analyzed, greatly reducing the chance of human error. The PCR machine also tends to heat up, so a cooling fan would help to keep the system cool and reduce any risks of burns.
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