Distance between the smart phone cradle and drop = 6.8 cm
Solutions Used for Calibration
Calibration via H2O
Calibration via the standard given which was a concentration 0.25 units of DNA per microliter |
Calibration via the standard given which was a concentration 0.5 units of DNA per microliter
Calibration via the standard given which was a concentration 1.0 units of DNA per microliter |
Calibration via the standard given which was a concentration 2.0 units of DNA per microliter
Calibration via the standard given which was a concentration 5.0 units of DNA per microliter |
Placing Samples onto the Fluorimeter
[Instructions: Step one, in your OWN words]
[Instructions: Step two, in your own words]
[Instructions: Step three, in your own words]
[Instructions: Step etc., in your own words]
Data Analysis
Representative Images of Negative and Positive Samples
Negative Sample
Positive Sample
Image J Values for All Calibrator Samples
Calibration curve
PCR Results Summary
Our positive control PCR result was ____ μg/mL
Our negative control PCR result was ____ μg/mL
Observed results
Patient _____ :
Patient _____ :
Conclusions
Patient _____ :
Patient _____ :
SNP Information & Primer Design
Background: About the Disease SNP
A nucleotide is composed of a nitrogenous base, a five carbon sugar, and at least one phosphate group. They are organic molecules that serve as monomers or sub units of nucleic acids, like DNA and RNA. Polymorphism is variations in coding and non coding DNA sequence among a population. It's a discontinuous genetic variation resulting in the occurrence of several different forms or types of individuals among the members of a single species. The SNP analyzed is found in the Homo Sapien species. The chromosome it is found in is 8:19956018. The clinical significance of the SNP is pathogenic. In addition, it is associated with the LPL (lipoprotein lipase) gene and linked to coronary heart disease. The functions of LPL are Apolipoprotein Binding, Heparin Binding, Lipoprotein Lipase Activity, Phospolipase activity, and Protein Binding. An allele is a variant form of a gene. Some genes have a variety of different forms, which are located at the same position on a chromosome. Disease associated allele is AGT. The numerical position of AGT is 19956018.
The diseased primer had no matches because the humane genome does not have the mutation of AGT. Therefore, the would be no match with the natural human genome tested against in the website. This variation is what makes each individual's makeup unique. The AGT mutation is what makes a person have coronary heart disease.