BME100 s2015:Group18 12pmL6

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR COMPANY Name: Walter C. Bregon Role(s): Chief of Neurosurgery Name: Abdurrahman Darwish Name: Jenna Taras Name: Nathan LeFort Name: Eyerusalem Name: Hau Nguyen LAB 6 WRITE-UP Bayesian Statistics Overview of the Original Diagnosis System Error prevention: • Using the black box to cover the sample to protect the fluorescence from interacting with light available in the room • Placing the drop in the middle of the light in order to give the most accurate picture • Dispose the tip of the Micropipette each time to prevent the samples from contamination Calculation Discussion: Using the data collected from the entire class, we used the following formula P(A|B) = ( P(B|A) * P(A) ) / ( P(B) ) based on the values given to us ( P(A),P(B) and P(B|A) ) for calculation 1 and followed the same procedure for calculations 2, 3 and 4. Sources of Error: • Rounding to the wrong decimal places or rounding off too soon in the calculations • Mixing the results of A and B or negative and positive • Miscounting the POS and negative values • some of data were inconclusive • error might have propagated from wrong data from the different groups What Bayes Statistics Imply about This Diagnostic Approach CALCULATION 1: Variable Description Numerical Value A Positive final test conclusion 0.4375 B Positive PCR reaction 0.4167 P(B/A) Probability of B given A 0.9 P(A/B) Probability of A given B 0.9449 CALCULATION 2: Variable Description Numerical Value A Negative final test conclusion 0.5625 B Negative PCR reaction 0.54167 P(B/A) Probability of B given A 0.923 P(A/B) Probability of A given B 0.958 CALCULATION 3: Variable Description Numerical Value A Patient Develops Disease 0.3529 B Positive Final Test 0.4411 P(B/A) Probability of B given A 0.8 P(A/B) Probability of A given B 0.651 CALCULATION 4: Variable Description Numerical Value A Patient NOT Develops Disease 0.647 B Negative Final Test 0.529 P(B/A) Probability of B given A 0.27 P(A/B) Probability of A given B 0.3302 Final Results: Which calculation describes the sensitivity of the system regarding the ability to detect the disease SNP? Calculation 3 Which calculation describes the sensitivity of the system regarding the ability to predict the disease? Calculation 1 Which calculation describes the specificity of the system regarding the ability to detect the disease SNP? Calculation 4 Which calculation describes the specificity of the system regarding the ability to predict the disease? Calculation 2 Computer-Aided Design TinkerCAD We assembled the original Open PCR machine using TinkerCAD. We placed every part and used the tools to align them and produce the final part using all the pieces. Then, we changed the color to our taste. We decided that the size was an issue because we wanted it to be portable. As such, we made the machine smaller using the transformation tools, and so it is portable. Our Design We chose this design because we liked the colors; we placed the fans and heating system near each other (near the vents) for better cooling. We put two fans and two heating systems as well as a larger power source to power it more efficiently. The size of the machine was reduced in order to make it portable and convenient. Feature 1: Consumables Kit Instead of having to get rid of the tips after every run, we will include a sanitizer to prevent cross-contamination between samples/solutions. We will include an improved micropipette design which includes three switchable/rotatable tubes to switch between different samples so that constant replacement of tips/micropipette is not necessary. This will make the entire process more efficient. As mentioned previously, our consumables packaging plan will reduce time-wasting and is more cost efficient as resources can be reused more often and less waste is produced as a result of cross-contamination worries. Feature 2: Hardware - PCR Machine & Fluorimeter The PCR machine will have one more heating unit and fan to cool the machine so it can run faster. The whole thing is smaller so it will be more portable. It also possesses a palatable array of colors. The covering process of the fluorimeter will be automated so that preventing light isn't as difficult. We redesigned the PCR machine to include an additional heating unit and fan near the open vents to improve the efficiency of the reactions. We also decreased the size to make the machine more portable. We also added color to the PCR machine make it more aesthetically pleasing and appealing to consumers. For the fluorimeter, we saw that the process of covering the drop and phone with a box to be inefficient and unwieldy. Therefore, we propose a new system where the box, already on top, has a button that, when pressed, covers the slides so that pictures can be taken with less energy. This ensures the SYBR-green will not be contaminated by light as easily.