BME100 s2015:Group17 12pmL5: Difference between revisions
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<!-- INSTRUCTIONS: (1) Show ONE image where you drew a circle around the droplet with the freehand tool for any sample with *no* DNA. (2) Show ONE image where you drew a circle around the droplet with the freehand tool for a sample *with* DNA (positive signal). -If you include more than two images, you will not receive any additional credit. --> | <!-- INSTRUCTIONS: (1) Show ONE image where you drew a circle around the droplet with the freehand tool for any sample with *no* DNA. (2) Show ONE image where you drew a circle around the droplet with the freehand tool for a sample *with* DNA (positive signal). -If you include more than two images, you will not receive any additional credit. --> | ||
*PART 1 | |||
[[Image:part1calibration.png]] | |||
*PART 2 | |||
[[Image:part2calibration.png]] | |||
'''Image J Values for All Calibrator Samples''' | '''Image J Values for All Calibrator Samples''' | ||
<!-- INSTRUCTIONS: Show a table for the ImageJ calf thymus DNA data. '''To save time on typing a new Wiki table from scratch''', use THIS TOOL to auto-generate a Wiki table: http://excel2wiki.net/wikipedia.php. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code. --> | <!-- INSTRUCTIONS: Show a table for the ImageJ calf thymus DNA data. '''To save time on typing a new Wiki table from scratch''', use THIS TOOL to auto-generate a Wiki table: http://excel2wiki.net/wikipedia.php. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code. --> | ||
*TABLE 1 | |||
[[Image:TABLE1CALIBRATION.png]] | |||
*TABLE2 | |||
[[Image:TABLE2CALIBRATION.png]] | |||
'''Calibration curve'''<br> | '''Calibration curve'''<br> | ||
<!-- INSTRUCTIONS: Place an image of your Excel plot with a line of best fit here. --> | <!-- INSTRUCTIONS: Place an image of your Excel plot with a line of best fit here. --> | ||
[[Image:CALIBRATIONGRAPH.png]] | |||
Revision as of 15:47, 1 April 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPProcedureSmart Phone Camera Settings
Data AnalysisRepresentative Images of Negative and Positive Samples
Image J Values for All Calibrator Samples
Calibration curve
Observed results
Conclusions
SNP Information & Primer DesignBackground: About the Disease SNP SNP, single nucleotide polymorphism is a DNA sequence variation occurring within a small population where a single base, A, T, C, or G differs between paired chromosomes or biological species. SNP are one of the most important genetic mutations that impact common disease. SNP results from replication errors and DNA damage, this phenomenon occurs exactly once in human evolution. Only sometimes does SNP have a correlation to a certain disease or trait. SNP has several applications in medicine such as gene discovery, allele mapping, and drug response prediction. Primer Design and Testing Results obtained from this lab were used to analyze the DNA sequence of two patients, one with a disease and the other without a disease. This lab demonstrated that primers bind to a certain region of a DNA sequence in order for the amplification of a small sample of DNA. Amplification occurs in two different directions on the DNA strand, the 5` and 3` ends. The 5` primers begins its sequence at the origin of the disease SNP variation location of 34370656. This was the location in the human genome where a thymine nucleotide has been mutated to a cytosine nucleotide, such a mutation results in the SNP disease that is seen in one of the patients. We used the UCSC In-Silico PCR website to test the non-disease primer to match the rs19956218 sequence.
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