BME100 s2015:Group16 12pmL5: Difference between revisions

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<!-- The type of smart phone you used and how you adjusted the camera settings, if applicable. If you used more than one phone, make an additional list. -->
<!-- The type of smart phone you used and how you adjusted the camera settings, if applicable. If you used more than one phone, make an additional list. -->
* Type of Smartphone: ZTE N9180
* Type of Smartphone: ZTE N9180
** Flash: No Flasj
** Flash: No Flash
** ISO setting:400
** ISO setting:400
** White Balance:Auto
** White Balance:Auto

Revision as of 14:44, 1 April 2015

BME 100 Spring 2015 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help


OUR TEAM

Name: Hanna Monroe
Name: Natkanes Pakjirasirikul
Name: Trevaun Walker
Name: Reaghan Fletcher
Name:Thomas Murphy


LAB 5 WRITE-UP

Procedure

Smart Phone Camera Settings

  • Type of Smartphone: ZTE N9180
    • Flash: No Flash
    • ISO setting:400
    • White Balance:Auto
    • Exposure:Highest
    • Saturation:Highest
    • Contrast:Lowest


Calibration

  • Distance between the smart phone cradle and drop = 6 cm


Solutions Used for Calibration

Initial Concentration of 2X Calf Thymus DNA solution (micrograms/mL) Volume of the 2X DNA Solution (μL) Volume of the SYBR GREEN I Dye Solution (μL) Final DNA concentration in SYBR Green I solution (μL)
5 80 80 2.5
2 80 80 1
1 80 80 0.5
0.5 80 80 0.25
0.25 80 80 0.125
0 80 80 0



Placing Samples onto the Fluorimeter

  1. [Instructions: Step one, in your OWN words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Analysis

Representative Images of Negative and Positive Samples


Image J Values for All Calibrator Samples


TABLE GOES HERE


Calibration curve


PCR Results Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL

Observed results

  • Patient _____ :
  • Patient _____ :

Conclusions

  • Patient _____ :
  • Patient _____ :




SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing