BME100 s2015:Group16 12pmL4: Difference between revisions
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<!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes into the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. --> | <!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes into the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. --> | ||
# Label Tubes with the labels from the table above (e.g. G16 +, G16 2-2...). | # Label Tubes with the labels from the table above (e.g. G16 +, G16 2-2...). | ||
# Transfer the + and - controls to the PCR tubes labeled G16 + and G16 - using the micropipette. | # Transfer 50 μL of the + and - controls to the PCR tubes labeled G16 + and G16 - using the micropipette. | ||
# Transfer corresponding patient DNA to special PCR tube using the micropipette. | # Transfer 50 μL of the corresponding patient's DNA to special PCR tube using the micropipette. | ||
# Dispose and replace the tips between each transfer. | # Dispose and replace the tips between each transfer. | ||
# Place the PCR reaction tubes in the thermal cycler. | # Place the PCR reaction tubes in the thermal cycler. |
Revision as of 12:59, 25 March 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
PCR Reaction Sample List
HEATED LID: 100°C
Research and DevelopmentPCR - The Underlying Technology Question 1: Question 2: Question 3: Question 4:
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