BME100 s2015:Group15 12pmL4

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Matt Birkholz Name: Tyler McCluskey Name: Kelsie Hermanson Name: Jasilynn Cox Name: student Role(s) LAB 4 WRITE-UP Protocol Materials Lab coat and disposable gloves PCR reaction mix, 8 tubes, 50 μL eachh: Mix contains Taq DNA polymerase, MgCl2, and dNTP’s (http://www.promega.com/resources/protocols/product-information-sheets/g/gotaqcolorless-master-mix-m714-protocol/) DNA/primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer A strip of empty PCR tubes Disposable pipette tips: only use each only once. Disposable pipette tips should never be reused or samples will be cross-contaminated Cup for discarded tips Micropipettor OpenPCR machine: shared by two groups PCR Reaction Sample List Tube Label PCR Reaction Sample Patient ID G15 + Positive control none G15 - Negative control none G15 1-1 Patient 1, replicate 1 50018 G15 1-2 Patient 1, replicate 2 50018 G15 1-3 Patient 1, replicate 3 50018 G15 2-1 Patient 2, replicate 1 69797 G15 2-2 Patient 2, replicate 2 69797 G15 2-3 Patient 2, replicate 3 69797 DNA Sample Set-up Procedure Materials mentioned above are obtained from the professors Two lengths of four tubes should be created by cutting the provided linked tubes in half. Tubes should be empty and will be used for the PCR reaction. The sides of the tubes should be labeled with a permanent marker then placed in a rack: G15 P (positive control), G15 N (negative control), G15 1-1, G15 1-2, G15 1-3, G15 2-1, G15 2-2, G15 2-3 For the positive control, 50 μL of PCR should be pipetted into the empty tube, along with the positive control DNA and DNA primers (50 μL). The total volume within the tube is now at 100 μL. New pipette tips should be utilized after every single transfer of liquids to avoid cross-contamination. OpenPCR program Research and Development PCR - The Underlying Technology