BME100 s2015:Group15 12pmL4

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Matt Birkholz Name: Tyler McCluskey Name: Kelsie Hermanson Name: Jasilynn Cox Name: student Role(s) LAB 4 WRITE-UP Protocol Materials Lab coat and disposable gloves PCR reaction mix, 8 tubes, 50 μL eachh: Mix contains Taq DNA polymerase, MgCl2, and dNTP’s (http://www.promega.com/resources/protocols/product-information-sheets/g/gotaqcolorless-master-mix-m714-protocol/) DNA/primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer A strip of empty PCR tubes Disposable pipette tips: only use each only once. Disposable pipette tips should never be reused or samples will be cross-contaminated Cup for discarded tips Micropipettor OpenPCR machine: shared by two groups PCR Reaction Sample List Tube Label PCR Reaction Sample Patient ID G15 + Positive control none G15 - Negative control none G15 1-1 Patient 1, replicate 1 50018 G15 1-2 Patient 1, replicate 2 50018 G15 1-3 Patient 1, replicate 3 50018 G15 2-1 Patient 2, replicate 1 69797 G15 2-2 Patient 2, replicate 2 69797 G15 2-3 Patient 2, replicate 3 69797 DNA Sample Set-up Procedure Step 1: Materials mentioned above are obtained from the professors Step 2: Two lengths of four tubes should be created by cutting the provided linked tubes in half. Tubes should be empty and will be used for the PCR reaction. Step 3: The sides of the tubes should be labeled with a permanent marker: G15 +, G15 -, G15 1-1, G15 1-2, G15 1-3, G15 2-1, G15 2-2, G15 2-3 Step 4: ... OpenPCR program Research and Development PCR - The Underlying Technology