OUR TEAM
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LAB 4 WRITE-UP
Protocol
Materials
- Lab coat and disposable gloves
- PCR reaction mix, 8 tubes, 50 μL eachh: Mix contains Taq DNA polymerase, MgCl2, and dNTP’s
(http://www.promega.com/resources/protocols/product-information-sheets/g/gotaqcolorless-master-mix-m714-protocol/)
- DNA/primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer
- A strip of empty PCR tubes
- Disposable pipette tips: only use each only once. Disposable pipette tips should never be reused or samples will be cross-contaminated
- Cup for discarded tips
- Micropipettor
- OpenPCR machine: shared by two groups
PCR Reaction Sample List
Tube Label |
PCR Reaction Sample |
Patient ID
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G15 + |
Positive control |
none
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G15 - |
Negative control |
none
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G15 1-1 |
Patient 1, replicate 1 |
50018
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G5 1-2 |
Patient 1, replicate 2 |
50018
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G15 1-3 |
Patient 1, replicate 3 |
50018
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G15 2-1 |
Patient 2, replicate 1 |
69797
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G15 2-2 |
Patient 2, replicate 2 |
69797
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G15 2-3 |
Patient 2, replicate 3 |
69797
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DNA Sample Set-up Procedure
- Step 1: Materials mentioned above are obtained from the professors
- Step 2:
- Step 3:
- Step 4: ...
OpenPCR program
Research and Development
PCR - The Underlying Technology
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