BME100 s2015:Group10 12pmL4: Difference between revisions
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| [[Image:Clayton Nunn.jpg|100px|thumb|Name: Clayton Nunn<br>Role(s)]] | | [[Image:Clayton Nunn.jpg|100px|thumb|Name: Clayton Nunn<br>Role(s)]] | ||
| [[Image:jpg|100px|thumb|Name: Isaac Clouse <br> Data Analysis)]] | | [[Image:Isaac D Clouse.jpg|100px|thumb|Name: Isaac Clouse <br> Data Analysis)]] | ||
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Revision as of 12:47, 25 March 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
OpenPCR program
Denature at 95°C for 30 seconds, Anneak at 57°C for 30 seconds, and Extend at 72°C for 30 seconds.
Research and DevelopmentPCR - The Underlying Technology Denaturation: The DNA strands are heated to a high temperature of about 95C. This weakens the hydrogen bonds holding the double helix together, at which point the bonds become so weak, the strands physically separate. The base pairs will be exposed and the DNA is ready for primers to be attached. Annealing: At a lower temperature, of about 55C, the primers are introduced into the mix. Since the DNA base pairs are exposed, the primers will be able to bind to specifically selected sites on the DNA. After this portion, the replication will begin. Extension: DNA polymerase begins to line up the new base pairs at a temperature varying with the polymerase. After a period of time, the DNA will have been doubled. The previous three steps will be repeated amny times in order to amplify a single section of the DNA to be copied millions and millions of times. Final Elongation: The DNA is put through one last extension to ensure all the strands have matching base pairs to form complete double helices. Final Hold: The DNA is stored a cool temperature to prevent it from denaturing again.
Credit: http://en.wikipedia.org/wiki/Polymerase_chain_reaction#Procedure
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