BISC219/F13:Gene Mapping

Background: Classical Forward Genetics and Gene Mapping
Lab 2: Examining mutant worms for mapping
Lab 3: Linkage Test Part 1
Lab 4: Linkage Test Part 2, Mapping and Complementation
Lab 5: Mapping con't; complete complementation
Lab 6: DNA sequencing analysis; Mapping con't
Lab 7: Complete Mapping: Score!

Schedule of Experiments for Series 2

Lab #	Dates	Activity	Outside Activity
2	9/11 - 9/17	Picking mutant worms for genetic mapping	Confirm mutant phenotype and set up first cross
3	9/18 - 9/24	Linkage crosses - heterozygous males with Unc hermaphrodites	Pick hermaphrodites for selfing
4	9/25 - 10/1	Examine phenotypes to determine linkage Mapping: Pick Unc non-Dpy to separate plates; Complementation: cross unknown Dpy worms to wild type males	Mapping: Pick putative double mutants Complementation: cross heterozygous males with known Dpy mutants
5	10/2 - 10/5 (10/15 Mon. Lab)	Mapping: Maintain double mutants (d u/d u): pick a few to individual plates Complementation: examine crosses for Dpy MALES	Nothing! Enjoy Fall Break!
6	10/16 - 10/22	DNA Sequencing Analysis:Examine sequencing data to determine the lesion in your gene of interest & significance in gene product; Mapping:Cross N2 males(++/++) with double mutant hermaphrodites (d u/ du)	Bioinformatics: Use Wormbase and BLAST to learn more about the gene in other species; Mapping:Cross herterozygous males (++/ d u) with L4 double mutant hermaphrodites (d u/ d u)
7	10/23 - 10/29	Complete Mapping SCORE! Find recombinantion frequency	Determine map distance between your Dpy and Unc marker