BE.109:Protein engineering: Difference between revisions
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'''[[BE.109:Protein engineering/Assessing beta-galactosidase | Day 2: Assessing beta-galactosidase]]''' | '''[[BE.109:Protein engineering/Assessing beta-galactosidase | Day 2: Assessing beta-galactosidase]]''' | ||
'''[[BE.109:Protein engineering/Purifying beta-galactosidase | Day 3: Purifying beta-galactosidase]]''' | |||
'''[[BE.109:Protein engineering/Possible Topics for Student Presentations | Day 4: Possible Topics for Student Presentations]]''' | '''[[BE.109:Protein engineering/Possible Topics for Student Presentations | Day 4: Possible Topics for Student Presentations]]''' | ||
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Revision as of 15:47, 28 December 2005
Module 2
Instructor: Natalie Kuldell
TA: Maria Foley
In this experimental module you will study an enzyme with a remarkable history, beta-galatosidase. "Beta-gal" as it's affectionately called had a starring role in the development of the operon model for gene regulation and continues to be a lab workhorse for gene expression studies. In bacteria, this enzyme hydrolyzes the disaccharide, lactose, into two simpler sugars, glucose and galactose. You will be measuring the efficiency of the enzymatic reaction using an artificial substrate, ONPG, which yields a yellow product when it is cleaved by beta-gal. Using a specialized bacterial strain, you will overexpress beta-gal to purify and analyze. Finally, you will test the effect of replacing a natural amino acids in beta-gal with an unnatural amino acid of your choosing, looking at the effect of such a substitution on the expression and activity of the modified enzyme.
Lab handouts
Day 1: Tools for Protein engineering
Day 2: Assessing beta-galactosidase