840:153g:Projects/project27/2012/11/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* A PCR was performed according to the protocol designed on 11/6, using ''M. trichosporium'' genomic DNA as template, and both primer sets. | * A PCR was performed according to the protocol designed on 11/6, using ''M. trichosporium'' genomic DNA as template, and both primer sets. | ||
* A ligation of the J23100 promoter and each of the two target vectors, pSB1K3 and J61002, was again attempted. This was performed at 4°C to test whether ligation temperature would increase the rate of successful ligation and therefore lead to the transformation of a functional plasmid-insert complex into ''E. coli''. | |||
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Latest revision as of 22:14, 26 September 2017
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