840:153g:Projects/project27/2012/11/13
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Failed PCR...Again
Lanes 1,6,13: Marker 2: 5μL template, mmoXY primers, 60.7°C anneal 3: 5μL template, mmoBZDC primers, 62°C anneal 4: 1:10 template dilution, mmoXY primers, 60.7°C anneal 5: 1:10 template dilution, mmoBZDC primers, 62°C anneal 7-9: mmoXY primers, anneal of 45°C, 47°C, and 50°C, respectively 10-12: mmoBZDC primers anneal of 45°C, 47°C, and 50°C, respectively 14: negative control (no primers used) 15: positive control (known template and primers) |