840:153g:Projects/project27/2012/10/16: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 9: | Line 9: | ||
* The pSB1K3 and J61002 plasmids were digested with Xba1 and Pst1 enzymes in order to remove inserted parts. pSB1K3 contained a 1022bp insert including parts R0010 (Lac1), B0034, E1010 (mRFP1), and B0015. J61002 contained a ~845bp insert including J23100, E1010 (mRFP1), and B0015. Digested plasmid DNA was run on a gel to separate plasmid backbone from insert DNA. Gel bands containing plasmid backbone were excised and stored for DNA recovery. | * The pSB1K3 and J61002 plasmids were digested with Xba1 and Pst1 enzymes in order to remove inserted parts. pSB1K3 contained a 1022bp insert including parts R0010 (Lac1), B0034, E1010 (mRFP1), and B0015. J61002 contained a ~845bp insert including J23100, E1010 (mRFP1), and B0015. Digested plasmid DNA was run on a gel to separate plasmid backbone from insert DNA. Gel bands containing plasmid backbone were excised and stored for DNA recovery. | ||
[[Image:Digest3-2.jpg| | [[Image:Digest3-2.jpg|400px]] | ||
Revision as of 19:43, 18 October 2012
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Plasmid Backbone Isolation
| |
