840:153g:Projects/project23/2012/10/25: Difference between revisions
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**Template dilution works well for either high or low concentrations. We will use 1:200 for further amplification. | **Template dilution works well for either high or low concentrations. We will use 1:200 for further amplification. | ||
**Annealing temperature did not seem to vary results for this range. | **Annealing temperature did not seem to vary results for this range. | ||
*After analyzing this gel, we decided to run another PCR to do a side-by-side comparison of the two fragments and the combined reaction, to see if they are separate products. We used only the 1:200 template, and the 65°C annealing temperature, as they gave us the cleanest images to date. We prepared an upstream and downstream reaction from our original total-DNA sample, and primered/ non-primered set identical to earlier this week. We also prepared each of these sets without template DNA for negative control. Lastly, we prepared 6 more primered samples to extract DNA from if we prove to have our intended product. We cycled our PCR with the same protocol we used in the past, but with a non-gradient annealing temperature. | |||
Revision as of 17:29, 29 October 2012
Cloning of the OmcF gene | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Continuation of the Mutagenesis Reaction
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