840:153g:Projects/project23/2012/10/25: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Casey Durnan (talk | contribs) |
Casey Durnan (talk | contribs) |
||
Line 8: | Line 8: | ||
==Continuation of the Mutagenesis Reaction == | ==Continuation of the Mutagenesis Reaction == | ||
*On Tuesday, we prepared to combine our upstream and downstream fragments in the final mutagenesis step. First a high concentration of 1:20 template DNA, and a low concentration of 1:200 were prepared with water for each the upstream and downstream fragments. We then prepared our samples to amplify. Our approach was to mix the fragments together with | *On Tuesday, we prepared to combine our upstream and downstream fragments in the final mutagenesis step. First a high concentration of 1:20 template DNA, and a low concentration of 1:200 were prepared with water for each the upstream and downstream fragments. We then prepared our samples to amplify. Our approach was to mix the fragments together with or without primers to try to identify an effective combination to create our entire gene. For each set we also investigated the possible effect of template concentration and annealing temperature, for a total of 12 different combinations. Our nomenclature used is three-part, and outlined below. | ||
*1.)Primer configuration | *1.)Primer configuration | ||
Line 53: | Line 55: | ||
*We ran these on a 1% agarose gel for 30min at 120V | *We ran these samples on a 1% agarose gel for 30min at 120V | ||
Revision as of 15:53, 29 October 2012
Cloning of the OmcF gene | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Continuation of the Mutagenesis Reaction
|