840:153g:Projects/project11/2010/11/04: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2010/11/04 Entry for 840:153g:Projects/project11) |
|||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Entry title== | ==Entry title== | ||
Monday: counted colonies from transformed bacteria plated last thursday. prepared media for onvernight growth, scraped of single colonies and placed into media. Incubated at 37 degrees overnight to grow liquid cultures. | |||
tuesday: plasmid mini prep to isolate plasmid from bacteria in preperation for digestion and Gel eletrophoresis. | |||
Thursday: digsted superpart promoter & rbs with EcoR1, Spe1 Superpart2 gene & double terminator with xball, pst1. ran on gel to confirm proper oriantation and base pair length of ligated parts. took picture | |||
next week plan to ligated the 2 confirmed superparts together. forming our completed part | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 13:56, 4 November 2010
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry titleMonday: counted colonies from transformed bacteria plated last thursday. prepared media for onvernight growth, scraped of single colonies and placed into media. Incubated at 37 degrees overnight to grow liquid cultures. tuesday: plasmid mini prep to isolate plasmid from bacteria in preperation for digestion and Gel eletrophoresis. Thursday: digsted superpart promoter & rbs with EcoR1, Spe1 Superpart2 gene & double terminator with xball, pst1. ran on gel to confirm proper oriantation and base pair length of ligated parts. took picture next week plan to ligated the 2 confirmed superparts together. forming our completed part | |
