840:153g:Projects/project11/2010/11/04
Project name | Main project page Previous entry Next entry |
Entry titleMonday: counted colonies from transformed bacteria plated last thursday. prepared media for onvernight growth, scraped of single colonies and placed into media. 10 tubes of superpart (promoter & rbs) 5 tubes- superpart (gene & double terminator). we took more of the first superpart due to concerns with ligation oriantation. Incubated at 37 degrees overnight to grow liquid cultures. tuesday: plasmid mini prep to isolate plasmid from bacteria in preperation for digestion and Gel eletrophoresis. Thursday: digsted superpart promoter & rbs with EcoR1, Spe1 Superpart2 gene & double terminator with xball, pst1. ran on gel to confirm proper oriantation and base pair length of ligated parts. took picture next week plan to ligated the 2 confirmed superparts together. forming our completed part |