7-AAD

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(categories)
Current revision (09:30, 5 May 2009) (view source)
m (categories)
 
Line 27: Line 27:
-->
-->
-
[[Category:Material]] [[Category:Dye]] [[Category:Cell]]
+
[[Category:Material]] [[Category:Dye]] [[Category:Viability dye]]

Current revision

7-aminoactinomycin-D

Contents

Purpose

  • Staining dead cells

Procurement

Use

  • 546/647
  • Nucleic acid dye
  • Often used in conjunction with Annexin-V when assaying for apoptotic cells
  • "The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labeled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Safety

References

  • MC O’Brien, et al. (1995) Cytometry 19:243
  • I Schmid et al. (1992) Cytometry 13:204
Personal tools