20.109(S13): Spring 2013 schedule

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| Thur/Fri Feb 7/8
| Thur/Fri Feb 7/8
| [http://web.mit.edu/be/people/runstadler.shtml JR]
| [http://web.mit.edu/be/people/runstadler.shtml JR]
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|  
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| [[User:Ian_Tay |Ian]]
| [[20.109(S13):Context-setting and primer design (Day1)| Context-setting and primer design]]
| [[20.109(S13):Context-setting and primer design (Day1)| Context-setting and primer design]]
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| Tue/Wed Feb 12/13  
| Tue/Wed Feb 12/13  
| [http://web.mit.edu/be/people/runstadler.shtml JR]
| [http://web.mit.edu/be/people/runstadler.shtml JR]
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| [[User:Ian_Tay |Ian]]
| [[20.109(S13):DNA extraction (Day2)| DNA extraction]]
| [[20.109(S13):DNA extraction (Day2)| DNA extraction]]
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| Thur/Fri Feb 14/15
| Thur/Fri Feb 14/15
| [http://web.mit.edu/be/people/runstadler.shtml JR]
| [http://web.mit.edu/be/people/runstadler.shtml JR]
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| [[User:Ian_Tay |Ian]]
| [[20.109(S13):PCR and paper discussion (Day3)| PCR and paper discussion]]
| [[20.109(S13):PCR and paper discussion (Day3)| PCR and paper discussion]]
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| Thur/Fri Feb 21/22
| Thur/Fri Feb 21/22
| [http://web.mit.edu/be/people/runstadler.shtml JR]
| [http://web.mit.edu/be/people/runstadler.shtml JR]
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| [[User:Ian_Tay |Ian]]
| [[20.109(S13):DNA cloning (Day4)| DNA cloning]]
| [[20.109(S13):DNA cloning (Day4)| DNA cloning]]
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| Tue/Wed Feb 26/27  
| Tue/Wed Feb 26/27  
| [http://web.mit.edu/be/people/runstadler.shtml JR]
| [http://web.mit.edu/be/people/runstadler.shtml JR]
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| [[User:Ian_Tay |Ian]]
| [[20.109(S13):DNA sequencing and primer analysis (Day5)| DNA sequencing and primer analysis]]
| [[20.109(S13):DNA sequencing and primer analysis (Day5)| DNA sequencing and primer analysis]]
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| Thur/Fri Feb 28/ Mar 1
| Thur/Fri Feb 28/ Mar 1
| [http://web.mit.edu/be/people/runstadler.shtml JR]
| [http://web.mit.edu/be/people/runstadler.shtml JR]
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| [[User:Ian_Tay |Ian]]
| [[20.109(S13):Journal club I (Day6)| Journal club I]]
| [[20.109(S13):Journal club I (Day6)| Journal club I]]
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| Tue/Wed Mar 5/6  
| Tue/Wed Mar 5/6  
| [http://web.mit.edu/be/people/runstadler.shtml JR]
| [http://web.mit.edu/be/people/runstadler.shtml JR]
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| [[User:Ian_Tay |Ian]]
| [[20.109(S13):Phylogenetic analysis (Day7)| Phylogenetic analysis]]
| [[20.109(S13):Phylogenetic analysis (Day7)| Phylogenetic analysis]]
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| Tue/Wed Mar 12/13
| Tue/Wed Mar 12/13
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]
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| [[User:Mark_Mimee |MM]]
|[[20.109(S13):Design mutant (Day1)| Design mutant]]
|[[20.109(S13):Design mutant (Day1)| Design mutant]]
|<br><font color = CC0000><b>Module 1 Draft Due</b></font color><br>
|<br><font color = CC0000><b>Module 1 Draft Due</b></font color><br>
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| Thur/Fri Mar 14/15
| Thur/Fri Mar 14/15
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]  
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]  
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|[[User:Mark_Mimee |MM]]
|[[20.109(S13):Site-directed mutagenesis (Day2)| Site-directed mutagenesis]]
|[[20.109(S13):Site-directed mutagenesis (Day2)| Site-directed mutagenesis]]
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| Tue/Wed Mar 19/20
| Tue/Wed Mar 19/20
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]
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|  
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| [[User:Mark_Mimee |MM]]
|[[20.109(S13):Bacterial amplification of DNA (Day3)| Bacterial amplification of DNA]]
|[[20.109(S13):Bacterial amplification of DNA (Day3)| Bacterial amplification of DNA]]
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| Thur/Fri Mar 21/22
| Thur/Fri Mar 21/22
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]  
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]  
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|[[User:Mark_Mimee |MM]]
|[[20.109(S13):Prepare expression system (Day4)| Prepare expression system]]
|[[20.109(S13):Prepare expression system (Day4)| Prepare expression system]]
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| Tue/Wed Apr 2/3
| Tue/Wed Apr 2/3
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]
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|  
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| [[User:Mark_Mimee |MM]]
|[[20.109(S13):Induce protein and evaluate DNA (Day5)| Induce protein and evaluate DNA]]
|[[20.109(S13):Induce protein and evaluate DNA (Day5)| Induce protein and evaluate DNA]]
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| Thur/Fri Apr 4/5
| Thur/Fri Apr 4/5
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]  
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]  
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|[[User:Mark_Mimee |MM]]
|[[20.109(S13):Purify protein (Day6)| Purify protein]]
|[[20.109(S13):Purify protein (Day6)| Purify protein]]
|<br><font color = CC0000><b>Module 1 Revision Due</b></font color><br>
|<br><font color = CC0000><b>Module 1 Revision Due</b></font color><br>
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| Tue/Wed Apr 9/10
| Tue/Wed Apr 9/10
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]
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| [[User:Mark_Mimee |MM]]
|[[20.109(S13):Characterize protein expression (Day7)| Characterize protein expression]]
|[[20.109(S13):Characterize protein expression (Day7)| Characterize protein expression]]
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| Thur/Fri Apr 11/12
| Thur/Fri Apr 11/12
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]  
| [http://web.mit.edu/be/people/jasanoff.shtml AJ]  
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|[[User:Mark_Mimee |MM]]
|[[20.109(S13):Assess protein function (Day8)| Assess protein function]]
|[[20.109(S13):Assess protein function (Day8)| Assess protein function]]
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| [[20.109(S13):Protein-level and wrap-up analysis (Day7)| Protein-level and wrap-up analysis]]
| [[20.109(S13):Protein-level and wrap-up analysis (Day7)| Protein-level and wrap-up analysis]]
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| <br><font color = CC0000><b>Module 3 Summary Due</b></font color><br>
|--
|--
| 3
| 3

Revision as of 21:35, 4 February 2013

20.109(S13): Laboratory Fundamentals of Biological Engineering

Home        Schedule Spring 2013        Assignments       
DNA Engineering        Protein Engineering        Cell Engineering              


MODULE DAY DATE LECTURER TA LAB NOTES
Tue/Wed Feb 5/6 ANS Orientation * Introductory Lecture (PDF)
* Intro Pre-Lab, T/R
1 1 Thur/Fri Feb 7/8 JR Ian Context-setting and primer design
1 2 Tue/Wed Feb 12/13 JR Ian DNA extraction
1 3 Thur/Fri Feb 14/15 JR Ian PCR and paper discussion
President's Day Feb 18 No lab Feb 19 or Feb 20
1 4 Thur/Fri Feb 21/22 JR Ian DNA cloning
1 5 Tue/Wed Feb 26/27 JR Ian DNA sequencing and primer analysis
1 6 Thur/Fri Feb 28/ Mar 1 JR Ian Journal club I
1 7 Tue/Wed Mar 5/6 JR Ian Phylogenetic analysis
1 8 Thur/Fri Mar 7/8 NO LECTURE TODAY Journal club II
2 1 Tue/Wed Mar 12/13 AJ MM Design mutant
Module 1 Draft Due
2 2 Thur/Fri Mar 14/15 AJ MM Site-directed mutagenesis
2 3 Tue/Wed Mar 19/20 AJ MM Bacterial amplification of DNA
2 4 Thur/Fri Mar 21/22 AJ MM Prepare expression system
Spring Break Mar 25-29 No lab all week
2 5 Tue/Wed Apr 2/3 AJ MM Induce protein and evaluate DNA
2 6 Thur/Fri Apr 4/5 AJ MM Purify protein
Module 1 Revision Due
2 7 Tue/Wed Apr 9/10 AJ MM Characterize protein expression
2 8 Thur/Fri Apr 11/12 AJ MM Assess protein function
Patriot's Day April 15-16 No lab Apr 16 or Apr 17
3 1 Thur/Fri Apr 18/19 ANS Design experiment
3 2 Tue/Wed Apr 23/24 ANS Initiate cell culture
Module 2 Report Due MONDAY APRIL 22 by 5 pm for all.
3 3 Thur/Fri Apr 25/26 ANS Testing cell viability
3 4 Tue/Wed Apr 30/May 1 ANS Preparing cells for analysis
3 5 Thur/Fri May 2/3 ANS Initiating transcript and protein assays
3 6 Tue/Wed May 7/8 ANS Transcript-level analysis
3 7 Thur/Fri May 9/10 ANS Protein-level and wrap-up analysis
Module 3 Summary Due
3 8 Tue/Wed May 14/15 ANS Student presentations
Thur May 16 No lab session. Evaluations during lecture, then celebratory lunch, room/time TBD
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