User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/16: Difference between revisions
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**[Au]=0.25mM | **[Au]=0.25mM | ||
**[HRP]=4.167uM | **[HRP]=4.167uM | ||
<br.> | |||
<u>Kinetics</u> | |||
*Run with H<sub>2</sub>O<sub>2</sub>, luminol, buffer solution, adding 100uL HRP-NPs to start reaction | |||
<br.> | |||
<u>Remake BSA-AuNPs</u> | |||
#See [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 08/28/13] for procedure | |||
#Calculations: | |||
#*Stock [HAuCl<sub>4</sub>]: 2.33mM | |||
#*Stock [BSA]: | |||
#*Final [HAuCl<sub>4</sub>]= 90×[BSA]=90×15.0uM=1350μM=1.350mM=final [HAuCl<sub>4</sub>] | |||
#**(2.33mM HAuCl<sub>4</sub> initial)(1mL solution)=(1.350mM HAuCl<sub>4</sub> final)(V) | |||
#**V=1.73mL BSA<br.> | |||
#*Combine 1mL HAuCl<sub>4</sub> and 1.73mL BSA, dilute with distilled water up to 10mL | |||
#Transfer solution to a test tube and cap with aluminum foil | |||
#Heat in oven at 80C | |||
#Transfer solution to a plastic falcon tube | |||
===Data=== | ===Data=== |
Revision as of 10:48, 16 October 2013
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October 16, 2013Objective
DescriptionReactions (Amounts in Cuvette)
<br.> Kinetics
<br.> Remake BSA-AuNPs
DataNotes |