Biomod/2013/Todai/Result: Difference between revisions
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<b>Agarose-gel electrophoresis to research the optimum time</b> | <b>Agarose-gel electrophoresis to research the optimum time</b> |
Revision as of 07:31, 31 August 2013
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<ul> <li><a href="http://openwetware.org/wiki/Biomod/2013/Todai">Home</a> </li> <li><a href="http://openwetware.org/wiki/Biomod/2013/Todai/Project">Project</a> </li> <li><a href="http://openwetware.org/wiki/Biomod/2013/Todai/Design">Design</a> </li> <li><a href="http://openwetware.org/wiki/Biomod/2013/Todai/Result">Result</a> </li> <li><a href="http://openwetware.org/wiki/Biomod/2013/Todai/Experiment">Experiment</a> <ul style="list-style-type: none;">
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<a href="http://openwetware.org/wiki/Biomod/2013/Todai/Experiment#Contents"> Contents</a></li> <li> <a href="http://openwetware.org/wiki/Biomod/2013/Todai/Experiment#PilotStudy"> Pilot Study</a></li> <li> <a href="http://openwetware.org/wiki/Biomod/2013/Todai/Experiment#Protocols"> Protocols</a></li> </ul> </li> <li><a href="http://openwetware.org/wiki/Biomod/2013/Todai/Team">Team</a> </li> <li><a href="http://openwetware.org/wiki/Biomod/2013/Todai/Sponsors">Sponsors</a> </li> </ul>
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<html> <head> <title>Result-Todai nanORFEVRE-</title> <style>
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<h1 class="big-title"><a name="Result"> Result</a></h1> <br> <div style="position:relative;left:30px;"> <h3>We focus on pilot study now, so please visit <a target="_blank" href="http://openwetware.org/wiki/Biomod/2013/Todai/Experiment#PilotStudy" style="color:#e00000"> Pilot Study </a> ,too. </h3> </div> <br>
<!--◆◆A.Cylinder in barrel by DNA origami◆◆-->
<h1 class="heading"><a name="A.Cylinder in barrel by DNA origami"> A. Cylinder in barrel by DNA origami</a></h1>
<!--◆◆1.BarrelMaking◆◆-->
<article> <h2 class="small-title"><a name="STEPA-1"> STEP1. DNA strands assemble to form designed structures.</a></h2>
<!--Method-->
<div class="zairyou-heading">[Method]</div> <figure class="figure-left"> <img style="float:left; margin:0;margin-right:-10px;margin-bottom:10px;" src="http://openwetware.org/images/7/79/BarrelSize-Todai.png" width="200px" height="200px" > </figure>
<div id ="step1Introduction"> <p class="paragraph"> First, the DNA nanostructure, <a target="_bramk" href="http://openwetware.org/wiki/Biomod/2013/Todai/Design#2.Cylinder in barrel by DNA origami" style="color:#e00000"> "Cylinder in barrel" </a> , was used for pilot study. </p>
<p class ="paragraph">
To know the optimum condition of the structure assembly, we did experiments in three conditions as follows. <ul style="position:relative;left:30px;"> <li>At different concentration of MgCl<sub>2</sub></li> <li>At different incubate temperature</li> <li>At different length of incubate time</li>
</ul> (<a target="_blank" href="http://openwetware.org/wiki/Biomod/2013/Todai/Experiment#Protocols" style="color:#e00000;"> protocols </a> ) </p> </div> <br> <br>
<!--Result&Discussion1-->
<div class="zairyou-heading">[Result & Discussion]</div>
<div class="res-conclusion"> To decide optimum concentration of MgCl<sub>2</sub> </div>
<figure> <center> <img src="http://openwetware.org/images/a/a9/Result-SA1-MgCl2-Todai-new.png" width=480px height=360px> <figcaption> <b>Agarose-gel electrophoresis to research the optimum concentration of MgCl<sub>2</sub></b> </figcaption> </center> </figure>
<p class="paragraph">
Fast-migrating species upon agarose-gel electrophoresis was yielded at 20mM MgCl<sub>2</sub> condition. Correctly folded structures were yielded at 20mM of MgCl<sub>2</sub>, because they were more compact than misfolded versions. Also, the structures at high concentration (30 and 40mM) of MgCl<sub>2</sub> tend to aggregate because of positive charge.
</p> <div class="res-conclusion"> --->> Optimum concentration of MgCl<sub>2</sub>: 20mM </div>
<br> <br>
<!--Result&Discussion2-->
<div class="res-conclusion"> To decide optimum incubate temparature </div> <figure> <center> <img src="http://openwetware.org/images/3/3b/Result-SA1-Temp-Todai-new.png" width=480px height=360px > <figcaption> <b>Agarose-gel electrophoresis to research the optimum temperature</b> </figcaption> </center> </figure>
<p class="paragraph">
Fast-migrating species upon agarose-gel electrophoresis was yielded at 47.7 or 51.6 degree C. The band at 47.7 degree C is more clear than that of at 51.6 degree C. The band of 47.7 degree C is fast migrated and strong.
</p> <div class="res-conclusion"> --->> Optimum temparature : 47.7 degree C </div> <br> <br>
<!--Result&Discussion2-->
<div class="res-conclusion"> To decide optimum length of incubate time </div>
<figure> <center> <img src="http://openwetware.org/images/5/5a/Result-StepA1-Time-Todai-newnew.jpg" width=640px height=360px > <figcaption> <b>Agarose-gel electrophoresis to research the optimum time</b> </figcaption> </center> </figure>
<p class="paragraph"> The structure assembled for 4h or longer are compact. The band for 4h is fast migrated and strong. </p>
<div class="res-conclusion"> --->> Optimum incubate time : 4h </div>
<br> </article> <br>
<!--◆◆2.溶液中、バレルで多量体できるか◆◆-->
<article> <h2 class="small-title"><a name="STEPA-2"> STEP2-5.</a></h2> <br> <div style="font-size:150%;font-weight:bolder;"> Coming Soon...!!! </div> </article> <br>
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