Haynes Lab:Notebook/CRISPR Editing/2016/05/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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<br><br> | <br><br> | ||
'''Plate 1'''<br> | |||
Luc14 | |||
:Reps 1-3 | |||
::H3K27me3, IgG, input | |||
:::Primer set 1, Primer set 2, Primer set 3 | |||
<br> | <br> | ||
[[Image:16.05.30 qPCR Plate 1 screen shot.png]] | [[Image:16.05.30 qPCR Plate 1 screen shot.png]] | ||
<br> | <br> | ||
Primer master mix for primer sets 1 and 3<br> | Primer master mix for primer sets 1, 2, and 3<br> | ||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''''' | | align="center" style="background:#f0f0f0;"|'''''' | ||
| align="center" style="background:#f0f0f0;"|'''1 wells''' | | align="center" style="background:#f0f0f0;"|'''1 wells''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''34''' | ||
|- | |- | ||
| 2x SYBR mix||7.5|| | | 2x SYBR mix||7.5||255 | ||
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| 750nmol F/R primer mix||3|| | | 750nmol F/R primer mix||3||102 | ||
|- | |- | ||
| PCR water||2.5|| | | PCR water||2.5||85 | ||
|- | |- | ||
| total||13|| | | total||13||442 | ||
|} | |} | ||
<br><br> | <br><br> | ||
<br><br> | <br><br> | ||
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<br><br> | <br><br> | ||
'''Redo nested PCR of the Luc14 g034 samples'''<br> | '''Redo nested PCR of the Luc14 g034 samples'''<br> | ||
Did it with the wrong primers the first time, need to redo with P149/176. | Did it with the wrong primers the first time, need to redo with P149/176. | ||
Latest revision as of 01:49, 27 September 2017
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05/30/2016qPCR for ChIP!
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