Haynes Lab:Notebook/CRISPR Editing/2016/05/30

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05/30/2016

qPCR for ChIP!
Primer sets

Primer set Target F primer R primer amplicon length
1g025P331 P329 205
2g031, g034P308P160202
3GAPDHP203P204



Plate 1
Luc14

Reps 1-3
H3K27me3, IgG, input
Primer set 1, Primer set 2, Primer set 3


Image:16.05.30 qPCR Plate 1 screen shot.png
Primer master mix for primer sets 1, 2, and 3

' 1 wells 34
2x SYBR mix7.5255
750nmol F/R primer mix3102
PCR water2.585
total13442






Redo nested PCR of the KAH228/g034 experiments
Did it with the wrong primers the first time, need to redo with P149/176.



Redo nested PCR of the Luc14 g034 samples
Did it with the wrong primers the first time, need to redo with P149/176.




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