McClean:Drug Concentrations

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Yeast Media Antibiotic Concentrations

  • G418:
    • Stock is 200 mg/mL (1000X)
    • Final concentration 200 ug/ml
    • Use 1ul stock per ml of media

(NOTE FROM EMILY: According to the note I found in the box with drug stocks in the -80 in 3005, the stock concentrations are 50mg/mL as of 05/17/24 for G418)

  • ClonNAT:
    • Stock is 50 mg/ml (1000X)
    • Final concentration 50 ug/mL
    • Use 1 ul stock per ml of media
  • HygromycinB:
    • Stock is 50 mg/mL (166X)
    • Final concentration 300 ug/mL
    • Use 6ul of stock per ml of media