Imported:YPM/Timecourses for fitting model

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Category:Yeast Pheromone Response Model Back to main model page



Receptor/G protein

  • Timecourse of Ste2 levels upon pheromone treatment in the presence and absence of cycloheximide in Fig 1A and 2A in Jenness and Spatrick. 1986 PMID 3015412
  • Timecourse of Ste2 internalization in the presence of pheromone (with cycloheximide treatment)in Fig 7C. Hicke et al. 1996 PMID 8565073
  • Timecourse of Ste2 internalization in the presence and absence of pheromone (with cycloheximide treatment). Hicke et al. 1998 PMID 9548714
  • Timercouse of G protein activation in response to pheromone treatment (with and without cycloheximide) in Fig 3a. Yi et al. 2003 PMID 12960402
  • Timecourse of Sst2 levels in the presence of pheromone (with and without cycloheximide) in fig 1b and 7b in Hao et al. 2003 PMID 12968019

MAPK cascade

  • Timecourse of Ste20 binding to Ste4 upon pheromone exposure in figure 1a in Leeuw et al. 1998 PMID 9428767
  • Timecourse of Fus3 activation in response to pheromone in fig 4c in Esch and Errede. 2002 PMID 12077316
  • Timecourse of Fus3 activity in response to pheromone in fig 2b in Qi and Elion 2005 PMID 15961405
    • gel not quantitated...
  • Timecourse of Fus3 activity in WT and ptp2Δptp3Δ cells in figure 3c, and Fus3 activity in WT, msg5Δ, and ptp2Δptp3Δmsg5Δ cells in figure 6c in Zhan et al. 1997 PMID 9224718
    • Note that these experiments were in Bar1+ cells, so it may not be straightforward to interpret the results.
  • Timecourse of Ste11 degradation in response to pheromone (or absence of pheromone) in fig 1d in Esch and Errede. 2002 PMID 12077316
  • Timecourse of Ste7 degradation in response to pheromone in fig 3d in Wang and Dohlman 2002 PMID 11864977
  • Timecourse of Ste7 degradation in response to pheromone in fig 9 in Wang et al. 2003 PMID 12668671
  • Timecourse of Fus3 levels in the presence and absence of pheromone (with cycloheximide) in 7 in Wang et al. 2006 PMID 16361346
  • Timecourse of Msg5 levels in the presence and absence of pheromone (with cycloheximide) in 7 in Wang et al. 2006 PMID 16361346
  • The recovery half-time for Fus3-GTP that has been photobleached at the shmoo tip is 0.32s (+/-0.8s). The half-time of recovery of Ste5-GFP at the shmoo tip is significantly longer (8.22s +/-1.3s), suggesting that Fus3 may rapidly dissociate from Ste5. van Drogen et al. 2001 PMID 11781566

Gene expression

  • Timecourse of Ste12 levels in the absence and presence of pheromone (with and without cycloheximide, and with deletion of either Kss1 or Fus3) in fig 2c, 3b, and 5d in Esch et al. 2006 PMID 17041188


Overall

  • Timecourse of relative change in phosphorylation levels of many phosporylation sites in response to pheromone in Gruhler et al. 2005 PMID 15665377