Imported:YPM/Kd Ste5 Ste7

Category:Parameters - Yeast Pheromone Response Model Back to main model page

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<modelParameter>Kd_Ste5_Ste7 = 0.101</modelParameter> μM

Source

• FCCS study

Trust

• medium

Additional Details

• Using FCS to estimate concentrations of Ste7 and Ste5, and FCCS to estimate the concentration of Ste7:Ste5, the apparent affinity of interaction of Ste7 and Ste7 is 118 nM. Maeder et al. 2007 PMID 17952059
  • This Kd represents the overall affinity of interaction via direct interaction and indirect interaction.
  • Because there aren't any clear indirect interactions that would cause association of Ste7 with Ste5, the actual Kd is likely on the order of 118 nM.

• Using FCS to estimate concentrations of Ste7 and Ste5, and FCCS to estimate the concentration of Ste7:Ste5, the apparent affinity of interaction of Ste7 and Ste5 is 63 nM in the absence of pheromone, and 104 nM two hours after pheromone treatment. Slaughter et al. 2007 PMID 18077328
  • This Kd represents the overall affinity of interaction via direct interaction and indirect interaction.
  • Assuming that the interaction isn't pheromone regulated (the data from Meader et al. 2007 PMID 17952059 suggests that it isn't), and thus the difference in the measured values is due to experimental error, the average effective Kd is 84 nM.
  • Because there aren't any clear indirect interactions that would cause association of Ste7 with Ste5, the actual Kd is likely on the order of 84 nM.

See Ste5/MAPK cascade interactions for more details.