February 5th
Procedure
- Prepare stock solutions
- Dissolve HAuCl4 in water to have a solution of 10mM
- Dissolve 250mg of the citrate sodium salt into 25mL of water
- In a beaker mix 50mL of water and a volume of the gold stock solution corresponding to 1.214*10^-2 moles of Gold
- Bring solution to a boil
- Add a 0.5mL of the citrate sodium solution and reflux for 30 minutes
- Note color change from dark blue to red
- Protein Gold Nanoparticles
- First I made the buffer solution. The pH was 5. I made it using 0.0003 moles of acetic acid 1M and 0.0006 moles of potassium acetate
- Going to make 10^-6M HRP solution in the buffer\
- we then filled up 100ml volumetric flask
- Take 1ml of the solution and add to 9ml of nanoparticles...take 100ul and add to 9.9mls of gold to make solutions
- one solution was 10^-7M protein in nanoparticles. The other was 10^-8
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