User:Zachary I. Mendel/Notebook/Zacks Notebook/2014/01/29

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January 29th

Procedure

  • Same thing as yesterday. Today we are going to make citrate nanoparticles and try and get them to be around 50nm.
  • Different solutions that we will create
    1. 2mL gold and 0.5mL citrate
    2. 2.5mL gold and 0.5mL citrate
    3. 2.75mL gold and 0.5mL citrate
    4. 3mL gold and 0.5mL citrate
    5. 3mL gold and 1.5mL citrate
    6. 10mL gold and 1.5mL citrate
  • what we actually created
    • 1) 1.236ml gold and 0.5 citrate
    • 2) 2.5ml gold and 0.5 citrate
    • 3) 2ml gold and 0.5 citrate
    • 4) 2ml gold and 1.5 citrate
    • 5) 2ml gold and 2.5 citrate
    • 6) 6ml gold and 1.5 citrate
    • 7) 2ml gold and 4ml citrate

Notes

  • we noticed that added citrate took up some of the gold into solution. The ones that had more gold and low citrate would precipitate out, while high citrate less gold would be in solution.

Results

The UV-Vis spectra of the solutions were obtained and the following graph represents the spectra: UV-Vis of Nanoparticle Solutions Danelmen.png


The UV-Vis data allowed to calculate particle size using this reference. The following table summarizes those results and the calculated size of the particles for each solution.

Solution no. 1 2 3 4 5 6 7
Aspr 0.146 0.29 0.268 0.191 0.294 0.507 0.29
A450 0.082 0.146 0.145 0.116 0.196 0.313 0.182
Aspr/450 1.780487805 1.98630137 1.848275862 1.646551724 1.5 1.619808307 1.593406593
Size (nm) 20-25 40-45 25-30 14-16 10 14-16 12.0-14.0

Solutions 1 and 5 were the only two not to form gold precipitate during nano particle synthesis. From the results, solution yields the best nano particle size and that ratio will be used throughout the semester for nano particle synthesis.