User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/11/06

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What we did today

Today we analyzed our lysozyme and myoglobin nano particles using UV-Vis. We then created new hemoglobin and lysozyme nanoparticles in different ratios than before.

Notes

Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions

Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png

  • only the 30 to 1 exhibited an absorbance peak where it was expected


Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions
Corrected Absorbance spectra of Myoglobin Au-NP solution .png

  • 15 to 1 is the only ratio that produced 730nm absorbance peak


GOLD NANO SETUP!!!

Table 1. Preparation of Hemoglobin Stock solution

Molecular weight (g/mol) 64500
mass myoglobin (g) 0.0106
volume solution (L) 0.01
Concentration (M) 1.64341E-05


Table 2. Preparation of Gold stock solution

Molecular weight Au (g/mol) 393.83
mass Au(g) 0.032
volume stock(L) 0.025
Concentration (M) 0.003250133


Table 3. Preparation of Lysozyme stock solution

Molecular weight (g/mol) 14307
mass lysozyme (g) 0.0717
volume solution (L) 0.05
Concentration (M) 0.00010028


Table 4. Preparation of Hemoglobin-AuNP solutions

Ratio Amount of gold added concentration hemoglobin (M) volume Hemoglobin (mL) Volume Water (mL)
30 0.384599609 8.33333E-06 2.535377358 2.080023032
50 0.384599609 0.000005 1.521226415 3.094173976
70 0.384599609 3.57143E-06 1.086590296 3.528810094
90 0.384599609 2.77778E-06 0.845125786 3.770274604
110 0.384599609 2.27273E-06 0.691466552 3.923933838
130 0.384599609 1.92308E-06 0.585087083 4.030313308
150 0.384599609 1.66667E-06 0.507075472 4.108324919
170 0.384599609 1.47059E-06 0.447419534 4.167980857
190 0.384599609 1.31579E-06 0.400322741 4.21507765
210 0.384599609 1.19048E-06 0.362196765 4.253203625


Table 5. Preparation of Lysozyme Au-NP solutions

Ratio Amount gold added (mL) Concentration Lysozyme (M) Volume lysozyme (mL) Volume water (mL)
5 0.384599609 0.00005 2.493019545 2.122380845
10 0.384599609 0.000025 1.246509773 3.368890618
15 0.384599609 1.66667E-05 0.831006515 3.784393876
20 0.384599609 0.0000125 0.623254886 3.992145504
25 0.384599609 0.00001 0.498603909 4.116796482
30 0.384599609 8.33333E-06 0.415503258 4.199897133
35 0.384599609 7.14286E-06 0.356145649 4.259254741
40 0.384599609 0.00000625 0.311627443 4.303772947
45 0.384599609 5.55556E-06 0.277002172 4.338398219
50 0.384599609 0.000005 0.249301955 4.366098436