User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/30

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Entry title

Today we created new nanoparticles with myoglobin and lysozyme in order to determine the optimal concentration for fiber formation.

Notes

Today we are making HRP and myoglobin and pepsin nanoparticles

Table 1. Preparation of Myoglobin-AuNP solutions

Ratio Myoglobin Amount of gold added(mL) Concentration Myoglobin(M) Volume Myoglobin(mL) Volume Water Myoglobin(mL)
5 0.494264558 0.00005 2 2.505735442
10 0.494264558 0.000025 1 3.505735442
15 0.494264558 1.66667E-05 0.666666667 3.839068775
20 0.494264558 0.0000125 0.5 4.005735442
25 0.494264558 0.00001 0.4 4.105735442
30 0.494264558 8.33333E-06 0.333333333 4.172402108
35 0.494264558 7.14286E-06 0.285714286 4.220021156
40 0.494264558 0.00000625 0.25 4.255735442
45 0.494264558 5.55556E-06 0.222222222 4.28351322
50 0.494264558 0.000005 0.2 4.305735442


Table 2. Preparation of all Lysozyme-AuNP solutions

Ratio Lysozyme Amount gold added (mL) Concentration Lysozyme(M) Volume Lysozyme (mL) Volume Water Lysozyme (mL)
30 0.494264558 8.33333E-06 1.611148649 2.894586793
50 0.494264558 0.000005 0.966689189 3.539046253
70 0.494264558 3.57143E-06 0.690492278 3.815243164
90 0.494264558 2.77778E-06 0.53704955 3.968685892
110 0.494264558 2.27273E-06 0.439404177 4.066331265
130 0.494264558 1.92308E-06 0.371803534 4.133931907
150 0.494264558 1.66667E-06 0.32222973 4.183505712
170 0.494264558 1.47059E-06 0.28432035 4.221415092
190 0.494264558 1.31579E-06 0.254391892 4.25134355
210 0.494264558 1.19048E-06 0.230164093 4.275571349


Table 3. Stock Solution concentrations

Concentration of Myoglobin (M) Concentration Gold(M) Concentration Lysozyme (M)
0.000125 0.00252901 2.58615E-05


Table 4. Preparation of Lysozyme Stock solution

Molecular Weight(g/mol) 14307
mass lysozyme(g) 0.0037
volume solution(mL) 10
Concentration Lysozyme (M) 2.58615E-05


Table 5. Preparation of Myoglobin Stock solution

Molecular weight (g/mol) 17600
mass myoglobin (g) 0.022
volume solution (mL) 0.01
Concentration (M) 0.000125